银纳米颗粒及其体外细胞毒性-真菌辅助合成

Vardhana Janakiraman, Kathiravan Govindarajan, Thenmozhi Thenmozhi
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摘要

纳米技术与纳米粒子的产生和稳定有关。这种生物方法需要制造出被能够消化各种形式的纳米颗粒的微生物吃掉的纳米颗粒。在本研究中,利用短拟盘孢真菌来证明细胞外生产稳定的纳米银。从一株稳定的玫瑰花(Catharanthus roseus, L)叶片样品中分离得到真菌培养物。它们是用AgNO3溶液在室温和黑暗条件下处理细胞滤液和真菌垫后产生的。(1毫米)。细胞滤液制备的银纳米颗粒大小在171 ~ 378 nm之间,而真菌生物量大小在140 ~ 280 nm之间。MCF-7和A549细胞系同样用真菌制造的银纳米颗粒处理。采用GraphPad Prism 5软件,根据IC50值跟踪不同浓度MCF-7和A549细胞系在24和48小时的活细胞百分比。
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Silver Nanoparticles and Its Invitro Cytotoxic Behaviour- A Fungi Aided Synthesis
Nanotechnology is concerned with the creation and stabilisation of nanoparticles. The biological method necessitates the creation of nanoparticles that are eaten by microorganisms capable of digesting nanoparticles in various forms. The fungus Pestaloptiopsis breviseta is used in this study to demonstrate the extracellular production of stable silver nanoparticles. The fungal culture was isolated from a stable Catharanthus roseus (L) G.don leaf sample, a common therapeutic plant. They were produced after the AgNO3 solution was employed to treat the cell filtrate and the fungal mat at room temperature and in the dark. (1 mM). The cell filtrate made silver nanoparticles that were between 171-378 nm in size, whereas the fungal biomass was between 140-280 nm in size. The cell lines MCF-7 and A549 were likewise treated with the silver nanoparticles made by the fungi. GraphPad Prism 5 software was used to track the percentage of living cells for 24 and 48 hours at different concentrations of the MCF-7 and A549 cell lines based on the IC50 value.
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