Association between Anti-Sperm Antibody and Intra-cytoplasmic Sperm Injection Outcomes Among Male Infertility Patients

Background: Infertility, a clinical and public concern, affects both social life and the health system. Sperm abnormalities which include (Oligozoospermia), (teratozoospermia) or abnormalities related to sperm motility are essential factors in male infertility. Anti-sperm antibodies (ASA) are the leading cause of immune infertility in men (autoimmune disease). Intra-cytoplasmic Sperm Injection (ICSI) is specially designed to manage severe cases of male-factor infertility. this study aimed to determine the effect of ASA on ICSI outcomes among Normospermia and (Oligozoospermia, Asthenozoospermia and Teratozoospermia). Material and methods: This is a cross-sectional study performed at Fertility Center, Najaf-Iraq between Jan to June 2023, it included 50 couples who suffered from a minimum of 12 months of primary fertility who had attended the Fertility Center. They were divided into four groups (Normospermia, Oligospermia, Teratozoospermia, and Asthenospermia) according to their seminal fluid analysis (SFA). Sperms were collected by ICSA, immobilized, and processed. The oocytes were collected, injected, and prepared for fertilization , after that fertilization took place. Finally, pregnancy was examined in each female partner. Results: there was no significant difference in the age of the four groups, there was no relationship between ASA and Sperm concentration, and there was no relationship between ASA and Normal Sperm Morphology, fertilization , & pregnancy rates, in addition to other ICSI outcomes, were similar among the four groups.in conclusion, pregnancy rates were higher in the normospermia group than the other three groups and ICSI overcomed the presence of ASA in the semen plasma.