Jana Žiarovská, Katarína Ražná, Lucia Urbanová, Lucia Klongová, Adam Kováčik, Matúš Kučka, Ľubomír Harenčár, Lucia Zeleňáková
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引用次数: 0
摘要
基于dna的标记指纹技术是为了有效地应用于植物基因组的鉴定或比较研究而发展起来的。在牛痘属中，DNA标记有助于解决它所包含的物种之间的分类关系。本研究以变应原编码基因和微rna作为DNA标记，应用于长叶苗(Vaccinium corymbosum L.)和myrtilus L.的指纹图谱表征。结果表明，这两种技术都能有效地区分所分析的两种vacinium种质资源，并产生多态性，以betv1 Based Amplicon polymorphism和Profilin Based Amplicon polymorphism为例，获得了myrtilus L的分离指纹图谱。
Allergen coding genes and miRNA-based DNA fingerprints in revealing differences among Vaccinium corymbosum L. and Vaccinium myrtillus L.
DNA-based marker fingerprinting techniques are developed with the aim of their effective use in the identification or comparative studies of plant genomes. In the genus Vaccinium, DNA markers help resolve taxonomic relationships among the species included in it. In this study, the allergen coding genes and micro RNAs were used as DNA markers and applied for the characterization of the fingerprints of Vaccinium corymbosum L. and Vaccinium myrtilus L. Both techniques were found to be effective in distinguishing the analysed Vaccinium accessions and in the generating of polymorphism and in the case of Bet v 1 Based Amplicon Polymorphism and Profilin Based Amplicon Polymorphism, separated fingerprints were obtained for Vaccinium myrtilus L.
期刊介绍：
- General agriculture - Animal science - Plant science - Environment in relation to agricultural production, land use and wildlife management - Agricultural economics and rural development
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