Babacar Faye, Soukeynatou Diabira, Omar Bassoum, Doudou G. M. Niang, Malick Ndiaye, Baye Karim Diallo, Alioune Dièye
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Material and method: This study was carried out on samples of biopsies of hypopharynx cancerous tissue (ulcerative-budding lesion) and healthy oropharyngeal tissue obtained from the ENT department of the Fann hospital, then sent to the Molecular Biology Unit of the Ouakam military hospital (HMO). The nucleic acids extraction was carried out using the standard method of the Zymo research kit “Quick-DNATM Miniprep Plus kit” https://www.zymoresearch.com/. Molecular HPV detection and genotyping were performed by multiplex RT-PCR with the Seegene AnyplexTM II HPV28 kit Detection on a Biorad CFX96 automaton according to the manufacturer’s protocol for the simultaneous genotyping of 28 types of HPV including 19 at High Risk (HR) and 9 low risk (LR). Results: 156 patients were sampled, 61 Hypopharynx cancer biopsies and 95 healthy tissues. The median age of the general population was 36.5 years [12, 73]; the median age of the population with hypopharyngeal cancer of 40 years. Of the general study population 24.36% (38/156) was infected with HPV. In populations with hypopharyngeal cancer, HPV prevalence was 19.67% (12/61), 17.84% (5/28) in men and 21.21% (7/33) in women. HPV6 was the most frequently encountered genotype in the cancer population. Multiple infections have also been noted in cancer patients: HPV6+HPV18, HPV6+HPV56. For patients without hypopharyngeal cancer, the HPV prevalence was 27.36% (26/95), 9.59% (7/73) in women and 89.36% (19/22) in men. Several types of HPV-HR genotypes (HPV18, HPV26, HPV69), and HPV-LR genotypes (HPV42, HPV43, HPV70, HPV6) have been detected in healthy patients but also cases of co-infections (HPV6+HPV69; HPV56+HPV44; HPV58+HPV18). Conclusion: Our results showed a higher prevalence of HPV in non-cancer patients compared to hypopharyngeal cancer patients. The genotypes (HPV 6, 18 and 56) were observed in the study population. 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The same year, 32,268 patients were hospitalized for this pathology, but 95% are associated with alcohol and tobacco poisoning. Few data exist on these cancers in Africa and Senegal. In recent years, many studies have hypothesized that about 25% of head and neck cancers are associated with high-risk oncogenic human papillomaviruses (HPV) whose role in cervical cancer was already widely established. Objective: To know the prevalence and genotypes of HPV in head and neck cancers, particularly hypopharyngeal cancer. Material and method: This study was carried out on samples of biopsies of hypopharynx cancerous tissue (ulcerative-budding lesion) and healthy oropharyngeal tissue obtained from the ENT department of the Fann hospital, then sent to the Molecular Biology Unit of the Ouakam military hospital (HMO). The nucleic acids extraction was carried out using the standard method of the Zymo research kit “Quick-DNATM Miniprep Plus kit” https://www.zymoresearch.com/. 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引用次数: 0
摘要
背景:近年来,头颈癌在世界范围内已成为常见病,发病率居第六位。2007年,法国的发病率增加了14,697人,其中男性增加了11,158人,排在第四位。同年,32,268名患者因这种病理住院,但95%与酒精和烟草中毒有关。关于非洲和塞内加尔这些癌症的数据很少。近年来,许多研究假设约25%的头颈部癌症与高危致瘤性人乳头瘤病毒(HPV)有关,HPV在宫颈癌中的作用已被广泛确立。目的:了解HPV在头颈部肿瘤,特别是下咽癌中的流行情况和基因型。材料与方法:本研究取材于范恩医院耳鼻喉科下咽癌组织(溃疡-出芽病变)和健康口咽组织活检标本,送至瓦卡姆军队医院分子生物学部。核酸提取采用zimo研究试剂盒“Quick-DNATM Miniprep Plus kit”https://www.zymoresearch.com/的标准方法进行。在Biorad CFX96自动机上使用Seegene AnyplexTM II HPV28试剂盒检测,采用多重RT-PCR进行分子HPV检测和基因分型,按照制造商的方案同时对28种HPV进行基因分型,其中19种为高风险(HR), 9种为低风险(LR)。结果:156例患者,下咽癌活检61例,健康组织95例。一般人群的中位年龄为36.5岁[12,73];下咽癌患者的中位年龄为40岁。在一般研究人群中,24.36%(38/156)感染了HPV。在下咽癌人群中,HPV患病率分别为19.67%(12/61)、17.84%(5/28)和21.21%(7/33)。HPV6是癌症人群中最常见的基因型。在癌症患者中也发现了多种感染:HPV6+HPV18, HPV6+HPV56。无下咽癌患者HPV患病率为27.36%(26/95),女性9.59%(7/73),男性89.36%(19/22)。几种HPV-HR基因型(HPV18、HPV26、HPV69)和HPV-LR基因型(HPV42、HPV43、HPV70、HPV6)已在健康患者中检测到,但也有合并感染的病例(HPV6+HPV69;HPV56 + HPV44;HPV58 + HPV18)。结论:我们的研究结果显示,与下咽癌患者相比,非肿瘤患者的HPV患病率更高。在研究人群中观察到基因型(HPV 6、18和56)。分子基因分型未显示HPV在下咽癌中的显著参与。
Molecular Genotyping of Human Papillomavirus (HPV) in Hypopharyngeal Cancer
Background: In recent years, head and neck cancers have become common worldwide, ranking sixth in incidence. In 2007, in France the incidence increased by 14,697 including 11,158 among men, which places them in fourth place. The same year, 32,268 patients were hospitalized for this pathology, but 95% are associated with alcohol and tobacco poisoning. Few data exist on these cancers in Africa and Senegal. In recent years, many studies have hypothesized that about 25% of head and neck cancers are associated with high-risk oncogenic human papillomaviruses (HPV) whose role in cervical cancer was already widely established. Objective: To know the prevalence and genotypes of HPV in head and neck cancers, particularly hypopharyngeal cancer. Material and method: This study was carried out on samples of biopsies of hypopharynx cancerous tissue (ulcerative-budding lesion) and healthy oropharyngeal tissue obtained from the ENT department of the Fann hospital, then sent to the Molecular Biology Unit of the Ouakam military hospital (HMO). The nucleic acids extraction was carried out using the standard method of the Zymo research kit “Quick-DNATM Miniprep Plus kit” https://www.zymoresearch.com/. Molecular HPV detection and genotyping were performed by multiplex RT-PCR with the Seegene AnyplexTM II HPV28 kit Detection on a Biorad CFX96 automaton according to the manufacturer’s protocol for the simultaneous genotyping of 28 types of HPV including 19 at High Risk (HR) and 9 low risk (LR). Results: 156 patients were sampled, 61 Hypopharynx cancer biopsies and 95 healthy tissues. The median age of the general population was 36.5 years [12, 73]; the median age of the population with hypopharyngeal cancer of 40 years. Of the general study population 24.36% (38/156) was infected with HPV. In populations with hypopharyngeal cancer, HPV prevalence was 19.67% (12/61), 17.84% (5/28) in men and 21.21% (7/33) in women. HPV6 was the most frequently encountered genotype in the cancer population. Multiple infections have also been noted in cancer patients: HPV6+HPV18, HPV6+HPV56. For patients without hypopharyngeal cancer, the HPV prevalence was 27.36% (26/95), 9.59% (7/73) in women and 89.36% (19/22) in men. Several types of HPV-HR genotypes (HPV18, HPV26, HPV69), and HPV-LR genotypes (HPV42, HPV43, HPV70, HPV6) have been detected in healthy patients but also cases of co-infections (HPV6+HPV69; HPV56+HPV44; HPV58+HPV18). Conclusion: Our results showed a higher prevalence of HPV in non-cancer patients compared to hypopharyngeal cancer patients. The genotypes (HPV 6, 18 and 56) were observed in the study population. Molecular genotyping does not show a significant involvement of HPV in hypopharyngeal cancer.