克里米亚-刚果出血热病毒在上几内亚共和国蜱虫中的传播

Mamadou Gando Diallo, Aissatou Boiro, Bonaventure Kolie, Ekaterina Naydenova, Abdoulaye Djibril Diallo, Souleymane Diallo, Thierno Amadou Labé Balde
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摘要

本研究的目的是绘制携带克里米亚-刚果出血热病毒(虫媒病毒)的媒介在上几内亚自然地区的分布图。坎坎县、达博拉县和法拉纳县被用作收集区。采用随机抽样的方法采集不同类型动物的生物材料。采用RT-PCR和ELISA两种分析方法。共采集蜱578只,分布在254个蜱池中,最常见的蜱属为无蜱属,有83个蜱池。RT-PCR检测病毒RNA阳性2例(0.8%)。直接酶联免疫吸附试验(ELISA)检测Ag仅产生1例阳性病例(0.4%)。结果表明,盖革棘头虫是上几内亚主要的媒介和宿主。
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Circulation of Crimean-Congo Haemorrhagic Fever Virus in Ticks in Upper Guinea-Republic of Guinea
The aim of this study was to map the distribution of agents carrying the Crimean-Congo haemorrhagic fever virus (arbovirus-tica) in the natural region of Upper Guinea. The prefectures of Kankan, Dabola and Faranah were used as collection areas. Random sampling of different types of animals was used to collect the biomaterial. Two types of analysis methods (RT-PCR and ELISA) were used. Out of a total of 578 ticks collected and divided into 254 pools, the genus Amblyomma was the most frequently encountered with 83 tick pools. Molecular analysis (RT-PCR) for the detection of virus RNA revealed 2 positive cases (0.8%). Direct enzyme-linked immunosorbent assays (ELISA) for the detection of Ag yielded only one positive case (0.4%). We found that the species Rhipicephalus geigyi was the main vector and reservoir of the pathogen in Upper Guinea.
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