含添加剂的DHB基质用于碳水化合物和糖缀合物的直接MALDI质谱分析

IF 0.2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Trends in Glycoscience and Glycotechnology Pub Date : 2023-03-25 DOI:10.4052/tigg.2214.1e

Hiroshi Hinou

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引用次数: 0

摘要

基质辅助激光解吸/电离(MALDI)是质谱(MS)中的软电离方法之一。它的分析物与基质的相容性可以提高分析物选择性电离效率和控制产物离子的破碎特性。2,5-二羟基苯甲酸(DHB)是一种多用途基质，可用于多种分子种类。虽然DHB通常被用作多糖MALDI质谱分析的首选基质，但也有许多尝试使用添加剂对DHB进行多糖分析的报道。本文将介绍以dhb为基础的基质添加剂，这些添加剂可以促进MALDI质谱对碳水化合物和糖缀合物的直接分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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DHB Matrix with Additives for Direct MALDI Mass Spectrometry of Carbohydrates and Glycoconjugates

Matrix-assisted laser desorption/ionization (MALDI) is one of the soft ionization methods in mass spectrometry (MS). Its analyte compatibility with a matrix can improve analyte-selective ionization efficiency and control the fragmentation properties of the product ions. 2,5-dihydroxybenzoic acid (DHB) is a versatile matrix that can be used for various molecular species. Although DHB is commonly used as the matrix of first choice for MALDI MS of glycans, many attempts to optimize DHB for glycan analysis using additives have also been reported. This mini-review will present additives of the DHB-based matrix that promote direct analysis of carbohydrates and glycoconjugates by MALDI MS.

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来源期刊

Trends in Glycoscience and Glycotechnology 生物-生化与分子生物学

CiteScore

0.40

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： TIGG is an international, bilingual journal covering glycoscience and glycotechnology and issued bimonthly since 1989. Since then editors are elected from academia and companies who agree to participate in a volunteer basis for the duration of four years. TIGG is an official journal of the Japanese Society of Carbohydrate Research. The journal covers the following categories. MINIREVIEWS describe recent progress in the fields of glycoscience and glycotechnology. Suggestions are always welcome in the form of a one-page synopsis (double spaced) addressed to the editor-in-chief. GLYCOTOPICS are short commentaries on the latest papers describing outstanding findings with background. GLYCODEBUTS introduce distinguished PhD theses or outstanding works of young scientists over the world in glycosciences and glycotechnologies. GLYCOFORUM is for our readers who wish to submit comments on scientific or social matters related to glycoscience and glycotechnology. LETTERS TO TIGG are for readers who wish to discuss contributions appearing in TIGG or matters related to FCCA.