Muhammed Mustafa POLAT, Ergun KAYA, İbrahim KIVRAK
{"title":"离体盐胁迫下培养的东方丹植物酚类化合物的比较色谱分析","authors":"Muhammed Mustafa POLAT, Ergun KAYA, İbrahim KIVRAK","doi":"10.21448/ijsm.1309982","DOIUrl":null,"url":null,"abstract":"In this study, the effects of boron salt stress on in vitro cultivated Liquidambar orientalis (L. orientalis), a relict-endemic plant species, and the resulting changes in its phenolic appearance were investigated. Salt stress can cause negative impact on plant growth and production, especially in species with low salinity and drought tolerance, affecting metabolite expression and somaclonal execution. To evaluate the effects of different boron salts on meristem regeneration and progression, clonal in vitro L. orientalis meristems were exposed to boric acid, sodium perborate, sodium metaborate, and disodium octaborate salts. When compared with the control group examples where salt application was not performed, the highest regeneration percentage was determined to be 100% with the application of 1 mg/L disodium octaborate. In terms of the shoot formation capacity index, it was determined to be 5 mg/L. With a value of 4.94, the application of sodium perborate yielded the best result. In L. orientalis plants, the greatest change in phenolic compounds due to boron salt applications was observed in the concentration of Quercetin with the sodium perborate salt application at 1 mg/L concentration.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":"25 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparative chromatographic analysis of phenolic compounds of Liquidambar orientalis plant cultivated under in vitro salt stress\",\"authors\":\"Muhammed Mustafa POLAT, Ergun KAYA, İbrahim KIVRAK\",\"doi\":\"10.21448/ijsm.1309982\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this study, the effects of boron salt stress on in vitro cultivated Liquidambar orientalis (L. orientalis), a relict-endemic plant species, and the resulting changes in its phenolic appearance were investigated. Salt stress can cause negative impact on plant growth and production, especially in species with low salinity and drought tolerance, affecting metabolite expression and somaclonal execution. To evaluate the effects of different boron salts on meristem regeneration and progression, clonal in vitro L. orientalis meristems were exposed to boric acid, sodium perborate, sodium metaborate, and disodium octaborate salts. When compared with the control group examples where salt application was not performed, the highest regeneration percentage was determined to be 100% with the application of 1 mg/L disodium octaborate. In terms of the shoot formation capacity index, it was determined to be 5 mg/L. With a value of 4.94, the application of sodium perborate yielded the best result. In L. orientalis plants, the greatest change in phenolic compounds due to boron salt applications was observed in the concentration of Quercetin with the sodium perborate salt application at 1 mg/L concentration.\",\"PeriodicalId\":14437,\"journal\":{\"name\":\"International Journal of Secondary Metabolite\",\"volume\":\"25 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Secondary Metabolite\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21448/ijsm.1309982\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Secondary Metabolite","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21448/ijsm.1309982","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
本研究研究了硼盐胁迫对离体栽培的残地植物——东方Liquidambar orientalis (L. orientalis)的影响及其对其酚类物质外观的影响。盐胁迫会对植物的生长和生产产生负面影响,特别是对低盐度和耐旱的物种,影响代谢物的表达和体细胞无性系的执行。为了研究不同硼盐对东方白羊草分生组织再生和进展的影响,在体外培养了硼酸盐、过硼酸钠盐、偏硼酸钠盐和辛酸二钠盐。与不施盐的对照组相比,施1mg /L辛酸二钠的再生率最高,为100%。在芽形成能力指标方面,确定其为5 mg/L。使用过硼酸钠的效果最好,其值为4.94。硼盐处理下,槲皮素和过硼酸钠在1 mg/L浓度下的含量变化最大。
Comparative chromatographic analysis of phenolic compounds of Liquidambar orientalis plant cultivated under in vitro salt stress
In this study, the effects of boron salt stress on in vitro cultivated Liquidambar orientalis (L. orientalis), a relict-endemic plant species, and the resulting changes in its phenolic appearance were investigated. Salt stress can cause negative impact on plant growth and production, especially in species with low salinity and drought tolerance, affecting metabolite expression and somaclonal execution. To evaluate the effects of different boron salts on meristem regeneration and progression, clonal in vitro L. orientalis meristems were exposed to boric acid, sodium perborate, sodium metaborate, and disodium octaborate salts. When compared with the control group examples where salt application was not performed, the highest regeneration percentage was determined to be 100% with the application of 1 mg/L disodium octaborate. In terms of the shoot formation capacity index, it was determined to be 5 mg/L. With a value of 4.94, the application of sodium perborate yielded the best result. In L. orientalis plants, the greatest change in phenolic compounds due to boron salt applications was observed in the concentration of Quercetin with the sodium perborate salt application at 1 mg/L concentration.