毒蕈碱受体作为卵巢癌节律治疗的靶点

Q4 Medicine Duzce Medical Journal Pub Date : 2023-09-22 DOI:10.18678/dtfd.1315416
Fatma SÖYLEMEZ, Çağatay Han TÜRKSEVEN
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引用次数: 0

摘要

目的:本研究首次探讨毒蕈碱类乙酰胆碱受体(mAChR)激动剂carbachol对顺铂耐药(A2780cis)和无顺铂(SKOV-3)卵巢癌细胞株增殖的影响,以进一步评价节律化疗的潜在治疗效果。材料与方法:采用xCELLigence实时细胞分析仪(RTCA)双平板(DP)系统检测苯酚对细胞增殖的抑制作用。初步研究确定100µM卡巴醇、1µM顺铂的剂量,100µM卡巴醇+ 1µM顺铂和100µM卡巴醇+ 10µM阿托品进行两组联合研究,以未用药的癌细胞为对照组。观察细胞增殖曲线96小时。采用RTCA 1.2.1软件,每隔1 h自动测定每孔细胞增殖抑制指数。 结果:顺铂联合给药可导致A2780cis和SKOV-3细胞系细胞数量呈时间依赖性减少(p
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Muscarinic Receptors as Targets for Metronomic Therapy in Ovarian Cancer
Aim: In this study, the effects of muscarinic acetylcholine receptor (mAChR) agonist carbachol on the proliferation of cisplatin-resistant (A2780cis) and cisplatin-free (SKOV-3) ovarian cancer cell line were for the first time investigated to further evaluate the potential therapeutic effect of metronomic chemotherapy. Material and Methods: The inhibitory effect of carbachol on cell proliferation was detected using the xCELLigence Real-Time Cell Analyzer (RTCA) dual plate (DP) system. A preliminary study was conducted to determine the dose of carbachol 100 µM, cisplatin 1 µM, and two combination studies were carried out with 100 µM carbachol + cisplatin 1 µM and 100 µM carbachol + 10 µM atropine, over cancer cells without drugs was used as the control group. The cell proliferation curve was monitored for 96 hours. The cell index value of inhibition in cell proliferation was automatically measured every hour for each well using RTCA 1.2.1 software. Results: Co-administration of carbachol with cisplatin caused a decrease in cell number in both A2780cis and SKOV-3 cell lines in a time-dependent manner (p
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来源期刊
Duzce Medical Journal
Duzce Medical Journal Medicine-Medicine (all)
CiteScore
0.60
自引率
0.00%
发文量
59
审稿时长
12 weeks
期刊最新文献
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