{"title":"柠檬酸对花蕙兰(Cymbidium floribundum)器官发生的影响","authors":"Anjum Ferdous ONA, Kazuhiko SHIMASAKI","doi":"10.2525/ecb.61.69","DOIUrl":null,"url":null,"abstract":"We examined the effects of citric acid (CA) on organogenesis from protocorm-like bodies (PLBs) of Cymbidium floribundum to determine the appropriate concentration of CA for organogenesis. Explants were cultured in modified MS media supplemented with various concentrations of CA and maintained at 25±1℃ and a 24 h light period for 9 weeks. Addition of 0.1 g/L CA in modified MS media partially reduced the browning intensity in the cultured PLBs, which significantly increased PLB formation, shoot formation from the PLBs, and root formation from the shoots. More than 0.1 g/L CA in modified MS media increased the browning intensity in cultured PLBs. We concluded that CA can effectively control the browning problem in PLB culture of C. floribundum, and addition of 0.1 g/L CA in modified MS media was optimum for organogenesis.","PeriodicalId":11762,"journal":{"name":"Environmental Control in Biology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Citric Acid on the Organogenesis of <i>Cymbidium floribundum</i>\",\"authors\":\"Anjum Ferdous ONA, Kazuhiko SHIMASAKI\",\"doi\":\"10.2525/ecb.61.69\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We examined the effects of citric acid (CA) on organogenesis from protocorm-like bodies (PLBs) of Cymbidium floribundum to determine the appropriate concentration of CA for organogenesis. Explants were cultured in modified MS media supplemented with various concentrations of CA and maintained at 25±1℃ and a 24 h light period for 9 weeks. Addition of 0.1 g/L CA in modified MS media partially reduced the browning intensity in the cultured PLBs, which significantly increased PLB formation, shoot formation from the PLBs, and root formation from the shoots. More than 0.1 g/L CA in modified MS media increased the browning intensity in cultured PLBs. We concluded that CA can effectively control the browning problem in PLB culture of C. floribundum, and addition of 0.1 g/L CA in modified MS media was optimum for organogenesis.\",\"PeriodicalId\":11762,\"journal\":{\"name\":\"Environmental Control in Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Control in Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2525/ecb.61.69\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Control in Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2525/ecb.61.69","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Effect of Citric Acid on the Organogenesis of <i>Cymbidium floribundum</i>
We examined the effects of citric acid (CA) on organogenesis from protocorm-like bodies (PLBs) of Cymbidium floribundum to determine the appropriate concentration of CA for organogenesis. Explants were cultured in modified MS media supplemented with various concentrations of CA and maintained at 25±1℃ and a 24 h light period for 9 weeks. Addition of 0.1 g/L CA in modified MS media partially reduced the browning intensity in the cultured PLBs, which significantly increased PLB formation, shoot formation from the PLBs, and root formation from the shoots. More than 0.1 g/L CA in modified MS media increased the browning intensity in cultured PLBs. We concluded that CA can effectively control the browning problem in PLB culture of C. floribundum, and addition of 0.1 g/L CA in modified MS media was optimum for organogenesis.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
