干皮质骨细胞外基质的拉伸力学性能：两种成骨不全症和一种健康对照髂骨活检组织的比较

IF 3.4 Q2 ENDOCRINOLOGY & METABOLISM JBMR Plus Pub Date : 2023-10-11 DOI:10.1002/jbm4.10826

Michael Indermaur, Daniele Casari, Tatiana Kochetkova, Bettina M. Willie, Johann Michler, Jakob Schwiedrzik, Philippe Zysset

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引用次数: 0

摘要

成骨不全症（OI）是一种与胶原蛋白有关的遗传性骨病，会增加骨折的发生率。然而，这种脆性机械行为的起源仍不清楚。成骨不全症骨骼的细胞外基质（ECM）显示出较高的骨矿化度（DBM），而 ECM 水平的压缩机械性能似乎并不低于健康骨骼。然而，胶原缺陷是否会改变 ECM 的拉伸特性尚不得而知。本研究旨在量化健康骨和 OI 骨 ECM 的拉伸特性。在三个经髂活检组织（健康 n = 1、OI I 型 n = 1、OI III 型 n = 1）中，制作了 23 个微拉试样（规格尺寸为 10 × 5 × 2 μm3），并在真空条件下进行准静态拉伸加载。结果提取了加载模量和极限强度。有趣的是，与对照组相比，OI 骨 ECM 的拉伸性能并不逊色。所有试样都显示出脆性破坏行为。根据矿化胶原纤维 (MCF) 的取向，将断裂面分为轴向、混合和横向断裂面类型 (FST)。此外，还从微计算机断层扫描（μCT）图像中提取了活检皮质的组织矿物质密度（TMD）。FST和TMD都是预测加载模量和极限强度的重要因素，调整后的R2分别为0.556（p = 2.65e-05）和0.46（p = 2.2e-04）。定量偏振拉曼光谱 (qPRS) 和位点匹配纳米压痕法进一步验证了 MCF 取向和 DBM 对邻近 ECM 力学性能的影响。从 qPRS 光谱中提取了 MCF 取向和 DBM，并建立了第二个力学模型来预测 MCF 取向和 DBM 的压痕模量（R2 = 67.4%，p = 7.73e-07）。两个 OI 髂嵴活检组织皮质骨 ECM 的拉伸力学性能并不比健康组织的低，而且主要取决于 MCF 取向和 DBM。© 2023 作者。JBMR Plus 由 Wiley Periodicals LLC 代表美国骨与矿物质研究学会出版。

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Tensile Mechanical Properties of Dry Cortical Bone Extracellular Matrix: A Comparison Among Two Osteogenesis Imperfecta and One Healthy Control Iliac Crest Biopsies

Osteogenesis imperfecta (OI) is a genetic, collagen-related bone disease that increases the incidence of bone fractures. Still, the origin of this brittle mechanical behavior remains unclear. The extracellular matrix (ECM) of OI bone exhibits a higher degree of bone mineralization (DBM), whereas compressive mechanical properties at the ECM level do not appear to be inferior to healthy bone. However, it is unknown if collagen defects alter ECM tensile properties. This study aims to quantify the tensile properties of healthy and OI bone ECM. In three transiliac biopsies (healthy n = 1, OI type I n = 1, OI type III n = 1), 23 microtensile specimens (gauge dimensions 10 × 5 × 2 μm³) were manufactured and loaded quasi-statically under tension in vacuum condition. The resulting loading modulus and ultimate strength were extracted. Interestingly, tensile properties in OI bone ECM were not inferior compared to controls. All specimens revealed a brittle failure behavior. Fracture surfaces were graded according to their mineralized collagen fibers (MCF) orientation into axial, mixed, and transversal fracture surface types (FST). Furthermore, tissue mineral density (TMD) of the biopsy cortices was extracted from micro–computed tomogra[hy (μCT) images. Both FST and TMD are significant factors to predict loading modulus and ultimate strength with an adjusted R² of 0.556 (p = 2.65e−05) and 0.46 (p = 2.2e−04), respectively. The influence of MCF orientation and DBM on the mechanical properties of the neighboring ECM was further verified with quantitative polarized Raman spectroscopy (qPRS) and site-matched nanoindentation. MCF orientation and DBM were extracted from the qPRS spectrum, and a second mechanical model was developed to predict the indentation modulus with MCF orientation and DBM (R² = 67.4%, p = 7.73e−07). The tensile mechanical properties of the cortical bone ECM of two OI iliac crest biopsies are not lower than the one from a healthy and are primarily dependent on MCF orientation and DBM. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.