伊拉克烧伤后患者FOXP3和CD25的表达水平

Q4 Biochemistry, Genetics and Molecular Biology Biomedicine (India) Pub Date : 2023-08-30 DOI:10.51248/.v43i4.3110
Zainab Thamer Showait AL-Asady
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引用次数: 0

摘要

调节性T细胞(Regulatory T cells, Treg)以CD25+表达和Foxp3+转录因子为特征,Foxp3+转录因子是这些细胞的特征标记。本研究旨在研究Foxp3在伊拉克巴格达烧伤后患者中的表达。材料与方法:研究样本38人,分为两组:第一组为烧伤总体表面积小于45% (TBSA 45%)和烧伤总体表面积大于45% (TBSA 45%)的患者。对照组为健康个体(n=10)。流式细胞术检测Treg细胞FOXP3和CD25的表达,并对所得数据进行统计学分析。结果:这项研究的结果显示,Foxp3和CD25表达显著增加Treg细胞中分离出患者外周血和回溯回溯45%和45%后第一天,第五天的烧伤病人与对照组相比,和Foxp3的表达水平(35.674,63.768,16.147)分别后的第一天烧,而Foxp3的表达水平在燃烧的第五天分别为(39.588,47.275,16.1470)。同样,TBSA45%组、TBSA45%组和对照组患者在第1天的CD5水平分别为29.747、43.447和16.793,而在第5天,三组患者的CD5水平分别为33.870.197和16.793。结论:烧伤患者Treg细胞Foxp3和CD25表达的升高在增强Treg细胞活性抑制免疫应答中起重要作用。
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The expression level of FOXP3 and CD25 in Iraqi patients with post burn injuries
Introduction and Aim: Regulatory T cells (Treg) are characterized by CD25+ expression and Foxp3+ transcription factors which is a characteristic marker for these cells. This study aimed to investigate the expression of Foxp3 in patients with post-burn injuries in Baghdad, Iraq. Materials and Methods: The study samples included 38 persons divided into two groups: the first group included patients with Total Body surface area (TBSA) of burn less than 45% (TBSA 45 %), and patients with TBSA of burn more than 45 % (TBSA 45%). The control group included healthy individuals (n=10). The expression of FOXP3 and CD25 of Treg cells was measured by using flow cytometry and data obtained was statistically analyzed. Results: The result of the study showed a significant increase in Foxp3 and CD25 expression in Treg cells that were isolated from peripheral blood in patients with TBSA 45 % and TBSA 45% following day 1 and day 5 of the burn injury in contrast to the control group, and the expression level of Foxp3 was (35.674, 63.768 ,16.147) respectively after the first day of the burn, while the expression level of Foxp3 after the fifth day of the burn was (39.588, 47.275, 16.1470) respectively. Similarly, the levels of CD5 recorded for patients in TBSA 45%, TBSA45% and control group on day1 was 29.747, 43.447 and 16.793 respectively, while on day 5 it was 33.870.197 and 16.793, for the three groups, respectively. Conclusion: The increase of Foxp3 and CD25 expression in Treg cells isolated from burn patients has an important effect in enhancing the activity of Treg cells in inhibiting the immune response.
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Biomedicine (India)
Biomedicine (India) Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
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