{"title":"脂质纳米颗粒的制备、表征和稳定性，包括不饱和脂质","authors":"Yeseul Park, Jin-Won Park","doi":"10.1515/tsd-2023-2544","DOIUrl":null,"url":null,"abstract":"Abstract Among the SARS-CoV-2 vaccines developed to date, the mRNA vaccines developed by the Pfizer/BioNTech and Moderna companies have been formulated using saturated lipids, specifically 1,2-distearoyl- sn -glycero-3-phosphocholine (DSPC), along with cholesterol. DSPC and cholesterol have the disadvantage of causing sclerosis. Therefore, in this study, lipid nanoparticles (LNPs) were prepared and characterized by replacing DSPC with 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) and excluding cholesterol. The results showed that the DOPC-based LNPs had a smaller diameter (75.67 nm) compared to the previous study and the polydispersity index (PI) indicated a good dispersion homogeneity, suggesting size uniformity. Additionally, the LNPs maintained their size between 57 nm and 152 nm and showed stable PI values (0.330–0.393) throughout the 25 days.","PeriodicalId":22258,"journal":{"name":"Tenside Surfactants Detergents","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preparation, characterization, and stability of lipid nanoparticles including unsaturated lipids\",\"authors\":\"Yeseul Park, Jin-Won Park\",\"doi\":\"10.1515/tsd-2023-2544\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Among the SARS-CoV-2 vaccines developed to date, the mRNA vaccines developed by the Pfizer/BioNTech and Moderna companies have been formulated using saturated lipids, specifically 1,2-distearoyl- sn -glycero-3-phosphocholine (DSPC), along with cholesterol. DSPC and cholesterol have the disadvantage of causing sclerosis. Therefore, in this study, lipid nanoparticles (LNPs) were prepared and characterized by replacing DSPC with 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) and excluding cholesterol. The results showed that the DOPC-based LNPs had a smaller diameter (75.67 nm) compared to the previous study and the polydispersity index (PI) indicated a good dispersion homogeneity, suggesting size uniformity. Additionally, the LNPs maintained their size between 57 nm and 152 nm and showed stable PI values (0.330–0.393) throughout the 25 days.\",\"PeriodicalId\":22258,\"journal\":{\"name\":\"Tenside Surfactants Detergents\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2023-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tenside Surfactants Detergents\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/tsd-2023-2544\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tenside Surfactants Detergents","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/tsd-2023-2544","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
摘要
在迄今为止开发的SARS-CoV-2疫苗中，由辉瑞/BioNTech和Moderna公司开发的mRNA疫苗使用饱和脂质(特别是1,2-二硬脂酰- sn -甘油-3-磷酸胆碱(dsc))和胆固醇配制。DSPC和胆固醇有引起硬化症的缺点。因此，本研究制备了脂质纳米颗粒(LNPs)，并用1,2-二油基- sn -甘油-3-磷酸胆碱(DOPC)取代dsc并排除胆固醇，对LNPs进行了表征。结果表明，dopc基LNPs的直径(75.67 nm)比之前的研究要小，多分散性指数(PI)显示出良好的分散均匀性，表明尺寸均匀。在25 d内，LNPs的尺寸保持在57 ~ 152 nm之间，PI值稳定在0.330 ~ 0.393。
Preparation, characterization, and stability of lipid nanoparticles including unsaturated lipids
Abstract Among the SARS-CoV-2 vaccines developed to date, the mRNA vaccines developed by the Pfizer/BioNTech and Moderna companies have been formulated using saturated lipids, specifically 1,2-distearoyl- sn -glycero-3-phosphocholine (DSPC), along with cholesterol. DSPC and cholesterol have the disadvantage of causing sclerosis. Therefore, in this study, lipid nanoparticles (LNPs) were prepared and characterized by replacing DSPC with 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) and excluding cholesterol. The results showed that the DOPC-based LNPs had a smaller diameter (75.67 nm) compared to the previous study and the polydispersity index (PI) indicated a good dispersion homogeneity, suggesting size uniformity. Additionally, the LNPs maintained their size between 57 nm and 152 nm and showed stable PI values (0.330–0.393) throughout the 25 days.
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Tenside Surfactants Detergents offers the most recent results of research and development in all fields of surfactant chemistry, such as: synthesis, analysis, physicochemical properties, new types of surfactants, progress in production processes, application-related problems and environmental behavior. Since 1964 Tenside Surfactants Detergents offers strictly peer-reviewed, high-quality articles by renowned specialists around the world.
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