桑树<i>抗氧化和抗增殖活性的植物化学筛选及评价(l)叶提取物抗乳腺癌细胞系

Q4 Pharmacology, Toxicology and Pharmaceutics Toxicology International Pub Date : 2023-09-20 DOI:10.18311/ti/2023/v30i3/33811
Richa Soni, Divya Gupta, Shamli Gupte, Arti Rathour, Sadhana Shrivastava, Sangeeta Shukla
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引用次数: 0

摘要

桑(L.)属于桑科,俗称白桑,在民间医学中用于治疗痢疾、利尿剂和抗糖尿病。桑属植物含有多种生物活性化合物,这是由桑属植物的生物学特性决定的。采用索氏提取法制备桑叶提取物,研究桑叶提取物的总酚含量、抗氧化活性和抗增殖活性。用Folin-Ciocalteu法测定总酚含量,用H2O2自由基清除法测定抗氧化能力。采用MTT(3-[4,5-二甲基噻唑-2-基]-2,5二苯基溴化四氮唑)法检测乳腺癌细胞株MCF-7和MDA-MB-231的抗增殖能力。结果表明,所有提取物均表现出明显的抗氧化和抗增殖活性,且呈剂量依赖性。其中,乙醇叶提取物具有较高的自由基清除活性以及对乳腺癌细胞的强细胞毒性。此外,对乙醇叶提取物的生物活性成分进行了分析,这可能是其有效的抗氧化和抗增殖潜力的原因。采用傅里叶变换红外(FTIR)技术分析了提取物中不同官能团的存在,并采用液相色谱-质谱(LC-MS)技术鉴定了提取物中的主要生物活性化合物。综上所述,桑葚具有良好的抗氧化活性和抗乳腺癌细胞增殖活性,可作为一种重要的天然抗氧化剂来源,用于开发抗各种疾病的药物。
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Phytochemical Screening and Evaluation of Antioxidant and Antiproliferative Potential of <i>Morus alba</i> (L.) Leaves Extracts Against Breast Cancer Cell Lines
Morus alba (L.) belongs to the family Moraceae commonly known as white mulberry used in folk medicine in the remedy of dysentery, diuretic, and antidiabetic treatments. Morus species contain several bioactive compounds which attribute to their biological properties. The purpose of this study is to determine the total phenolic content, antioxidant potential and antiproliferative activity of different leaf extracts of Morus alba (L). The plant leaves extracts were prepared by the Soxhlet extraction method. The total phenolic content was determined by Folin–Ciocalteu method and antioxidative potential was assessed by H2O2 free radical scavenging assay. The antiproliferative potential was investigated by MTT (3-[4,5- dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay using breast cancer cell lines MCF-7 and MDA-MB-231. Our results showed that all the extracts exhibited significant antioxidant and antiproliferative activity dose-dependently. Amongst all, the ethanolic leaf extract possessed higher radical scavenging activity as well as potent cytotoxicity against breast cancer cells. Furthermore, the ethanolic leaf extract was subjected to analyse of its bioactive composition that might be responsible for its potent antioxidant and antiproliferative potential. The presence of different functional groups in the extract was analysed by Fourier Transform Infrared (FTIR) technique and the main bioactive compounds in the extract were identified by Liquid Chromatography-Mass Spectrometry (LC-MS). Therefore, it can be concluded by the above findings that Morus alba (L.) has promising antioxidant activity and potent antiproliferative activity against breast cancer cells and thus can be used as a great source of natural antioxidants in developing drugs against various diseases.
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来源期刊
Toxicology International
Toxicology International Environmental Science-Health, Toxicology and Mutagenesis
CiteScore
0.60
自引率
0.00%
发文量
23
期刊介绍: Toxicology International is a peer-reviewed International Research Journal published bi-annually by the Society of Toxicology, India. The Journal is concerned with various disciplines of Toxicology including man, animals, plants and environment and publishes research, review and general articles besides opinions, comments, news-highlights and letters to editor.
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