{"title":"辐射诱导小鼠睾丸氧化应激及其毒性及堇青花提取物的预防作用","authors":"Priyanka Sharma , Jyoti Parmar , Preeti Verma , P.K. Goyal","doi":"10.1016/j.jrhm.2015.01.005","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p><span>Reproductive dysfunctions induced by various environmental toxicants are the prime concern in the today's changing global scenario. Living systems are constantly exposed to ionizing radiation<span> that cause cellular as well as genetic alterations leading to mutations and cell death. To evaluate the deleterious effects of low dose of gamma radiation on testicular tissue and their possible inhibition by </span></span><span><em>Tinospora cordifolia</em></span> root extract (TCE).</p></div><div><h3>Methods</h3><p>One group of Swiss albino mice was exposed to 2.5<!--> <!-->Gy gamma radiation to serve as the irradiated control, while the other group received TCE (75<!--> <!-->mg/kg b. wt./day) orally for 5 consecutive days half an hour before irradiation to serve as experimental.</p></div><div><h3>Results</h3><p>Irradiated animals experienced more severe testicular histopathological lesions and a considerable depletion in different spermatogenic cell counts as compared to that of normal animal. Furthermore, TCE pretreatment effectively prevented radiation-induced alterations in body weight, tissue weight, weight index, tubular diameters and anti oxidative parameter viz. lipid peroxidation<span><span>, glutathione and </span>catalase<span> activity in testes and restored almost a normal structure of testes.</span></span></p></div><div><h3>Conclusion</h3><p><em>T. cordifolia</em><span> root extract can be potentially used as an effective radio-protector against radiation induced testicular injuries in mammals.</span></p></div>","PeriodicalId":91915,"journal":{"name":"Journal of reproductive health and medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jrhm.2015.01.005","citationCount":"3","resultStr":"{\"title\":\"Radiation induced oxidative stress and its toxicity in testes of mice and their prevention by Tinospora cordifolia extract\",\"authors\":\"Priyanka Sharma , Jyoti Parmar , Preeti Verma , P.K. Goyal\",\"doi\":\"10.1016/j.jrhm.2015.01.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p><span>Reproductive dysfunctions induced by various environmental toxicants are the prime concern in the today's changing global scenario. Living systems are constantly exposed to ionizing radiation<span> that cause cellular as well as genetic alterations leading to mutations and cell death. To evaluate the deleterious effects of low dose of gamma radiation on testicular tissue and their possible inhibition by </span></span><span><em>Tinospora cordifolia</em></span> root extract (TCE).</p></div><div><h3>Methods</h3><p>One group of Swiss albino mice was exposed to 2.5<!--> <!-->Gy gamma radiation to serve as the irradiated control, while the other group received TCE (75<!--> <!-->mg/kg b. wt./day) orally for 5 consecutive days half an hour before irradiation to serve as experimental.</p></div><div><h3>Results</h3><p>Irradiated animals experienced more severe testicular histopathological lesions and a considerable depletion in different spermatogenic cell counts as compared to that of normal animal. Furthermore, TCE pretreatment effectively prevented radiation-induced alterations in body weight, tissue weight, weight index, tubular diameters and anti oxidative parameter viz. lipid peroxidation<span><span>, glutathione and </span>catalase<span> activity in testes and restored almost a normal structure of testes.</span></span></p></div><div><h3>Conclusion</h3><p><em>T. cordifolia</em><span> root extract can be potentially used as an effective radio-protector against radiation induced testicular injuries in mammals.</span></p></div>\",\"PeriodicalId\":91915,\"journal\":{\"name\":\"Journal of reproductive health and medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.jrhm.2015.01.005\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of reproductive health and medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214420X15000066\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of reproductive health and medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214420X15000066","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
摘要
在当今不断变化的全球环境中,各种环境毒物引起的生殖功能障碍是人们最关心的问题。生命系统不断暴露在电离辐射下,导致细胞和基因改变,导致突变和细胞死亡。目的探讨低剂量伽玛辐射对睾丸组织的有害影响及堇青花根提取物(TCE)的抑制作用。方法采用2.5 Gy γ射线照射瑞士白化病小鼠作为照射对照,另一组小鼠在照射前半小时口服TCE (75 mg/kg b. wt./d),连续5 d作为实验。结果与正常动物相比,辐射动物睾丸组织病理病变更严重,不同生精细胞计数明显减少。此外,TCE预处理能有效地预防辐射引起的睾丸体重、组织重量、体重指数、管径及抗氧化指标(脂质过氧化、谷胱甘肽和过氧化氢酶活性)的改变,使睾丸结构基本恢复正常。堇叶提取物可作为一种有效的辐射保护剂,用于防止哺乳动物睾丸受到辐射损伤。
Radiation induced oxidative stress and its toxicity in testes of mice and their prevention by Tinospora cordifolia extract
Background
Reproductive dysfunctions induced by various environmental toxicants are the prime concern in the today's changing global scenario. Living systems are constantly exposed to ionizing radiation that cause cellular as well as genetic alterations leading to mutations and cell death. To evaluate the deleterious effects of low dose of gamma radiation on testicular tissue and their possible inhibition by Tinospora cordifolia root extract (TCE).
Methods
One group of Swiss albino mice was exposed to 2.5 Gy gamma radiation to serve as the irradiated control, while the other group received TCE (75 mg/kg b. wt./day) orally for 5 consecutive days half an hour before irradiation to serve as experimental.
Results
Irradiated animals experienced more severe testicular histopathological lesions and a considerable depletion in different spermatogenic cell counts as compared to that of normal animal. Furthermore, TCE pretreatment effectively prevented radiation-induced alterations in body weight, tissue weight, weight index, tubular diameters and anti oxidative parameter viz. lipid peroxidation, glutathione and catalase activity in testes and restored almost a normal structure of testes.
Conclusion
T. cordifolia root extract can be potentially used as an effective radio-protector against radiation induced testicular injuries in mammals.