纳米AgO对嗜热枯草芽孢杆菌Ag-PQ发酵纤维素酶活性的增强作用。

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-11-29 DOI:10.1186/s43141-023-00619-1
Saddam Hussain, Muhammad Talha Yasin, Khurshid Ahmad, Suleman Khan, Rasheed Ahmad, Jallat Khan, Abdul Ghani, Muhammad Musaddiq Shah, Muzzamil Ahmed, Hasnat Tariq, Hamid Rehman, Adil Hussain, Muhammad Faheem, Syed Ali Imran Bokhari
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引用次数: 0

摘要

背景:纤维素酶是一种重要的生物加工酶,广泛应用于各个工业领域。本研究旨在提高枯草芽孢杆菌AG-PQ菌株纤维素酶的生物降解活性。为此,分别以AgNO3和FeSO4·7H2O盐为原料,采用基于五大步骤的水热法制备了AgO和FeO NPs;选择研究级材料,优化温度,pH,离心,样品用蒸馏水洗涤,在优化温度下在烘箱中完全干燥,最后研磨进行表征。通过扫描电镜(SEM)、能谱x射线(EDX)和x射线衍射(XRD)对合成的纳米粒子进行表征,分别确定了样品的形貌、元素组成和结构。扫描电镜记录了NPs的直径在70 ~ 95 nm之间。结果:优化了纤维素酶的最佳培养参数,培养时间为56 h,接种量为5%,椰粕为1%,硝酸铵为0.43%,pH为8,温度为37℃。在50 ppm浓度下,AgO NPs对纤维素酶活性有增强作用(57.804 U/ml/min),而FeO NPs对纤维素酶活性均有抑制作用。分子对接分析也被用来了解纳米催化剂提高酶活性的潜在机制。结论:本研究证实AgO NPs是提高耐热纤维素酶生物降解活性的较好的纳米催化剂,具有潜在的用于生物乙醇生产的能力。
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Enhancement effect of AgO nanoparticles on fermentative cellulase activity from thermophilic Bacillus subtilis Ag-PQ.

Background: Cellulase is an important bioprocessing enzyme used in various industries. This study was conducted with the aim of improving the biodegradation activity of cellulase obtained from the Bacillus subtilis AG-PQ strain. For this purpose, AgO and FeO NPs were fabricated using AgNO3 and FeSO4·7H2O salt respectively through a hydro-thermal method based on five major steps; selection of research-grade materials, optimization of temperature, pH, centrifuge, sample washed with distilled water, dry completely in the oven at the optimized temperature and finally ground for characterization. The synthesized NPs were characterized by scanning electron microscope (SEM), energy dispersive X-ray (EDX), and X-ray diffraction (XRD) to confirm the morphology, elemental composition, and structure of the sample respectively. The diameter of the NPs was recorded through SEM which lay in the range of 70-95 nm.

Results: Cultural parameters were optimized to achieve better cellulase production, where incubation time of 56 h, inoculum size of 5%, 1% coconut cake, 0.43% ammonium nitrate, pH 8, and 37 °C temperature were found optimal. The enhancing effect of AgO NPs was observed on cellulase activity (57.804 U/ml/min) at 50 ppm concentration while FeO NPs exhibited an inhibitory effect on cellulase activity at all concentrations. Molecular docking analysis was also performed to understand the underlying mechanism of improved enzymatic activity by nanocatalysts.

Conclusion: This study authenticates AgO NPs as better nanocatalysts for improved thermostable cellulase biodegradation activity with the extraordinary capability to be potentially utilized in bioethanol production.

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