circHIPK3/miR-378a-3p/HDAC4轴与骨质疏松性骨折的关联:一项全面的研究。

IF 1.6 4区 医学 Journal of Orthopaedic Surgery Pub Date : 2023-09-01 DOI:10.1177/10225536231219637
Lei Wang, Zhen Sheng, Tao Yao
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引用次数: 0

摘要

背景:骨质疏松性骨折(OFs)是一个重要的公共卫生问题,可导致疼痛和活动能力受损。OFs的潜在机制尚不清楚,但最近的研究表明circRNA-miRNA-mRNA通路可能起着至关重要的作用。目的:本研究旨在探讨circHIPK3/miR-378a-3p/HDAC4通路在OFs发病机制中的潜在参与。方法:收集10例OFs患者和10例健康对照者的组织和血清样本。通过qPCR和WB检测circHIPK3、miR-378a-3p和HDAC4的表达水平。此外,我们使用ELISA定量测定血清骨代谢相关指标(ALP、OC、TRAP、OCIF、ODF)水平。结果:我们的研究结果显示,与对照组相比,来自of患者的组织和血清样本中的circHIPK3和HDAC4均显著上调。同时,我们检测到miR-378a-3p在of组组织和血清中的表达水平低于对照组。此外,骨代谢相关指标ALP、TRAP、OCIF、ODF在of患者血清中的水平明显高于对照组。有趣的是,of患者的血清OCIF水平低于对照组。结论:我们的研究为circHIPK3/miR-378a-3p/HDAC4通路参与OFs的发病机制提供了重要证据。在of患者中观察到的circHIPK3和HDAC4的上调以及miR-378a-3p的下调提示它们对骨代谢的潜在调节作用。同时,血清骨代谢相关指标的异常表达可能通过破坏骨重塑平衡而促进OFs的发生。
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Association between circHIPK3/miR-378a-3p/HDAC4 axis and osteoporotic fractures: A comprehensive investigation.

Background: Osteoporotic fractures (OFs) are a significant public health issue, which can lead to pain and impaired mobility. The underlying mechanisms of OFs remain unclear, but recent studies have suggested that the circRNA-miRNA-mRNA pathway may play a crucial role.

Purpose: This study aimed to investigate the potential involvement of the circHIPK3/miR-378a-3p/HDAC4 pathway in the pathogenesis of OFs.

Methods: We collected tissue and serum samples from 10 patients with OFs and 10 healthy controls. The expression levels of circHIPK3, miR-378a-3p, and HDAC4 were measured by qPCR and WB. Additionally, we quantified the serum levels of bone metabolism-related indicators (ALP, OC, TRAP, OCIF, ODF) using ELISA.

Results: Our results revealed significant upregulation of circHIPK3 and HDAC4 in both tissue and serum samples from OF patients compared with controls. Simultaneously, we detected a lower expression level of miR-378a-3p in OF tissues and serum than that in the control group. Furthermore, the serum levels of bone metabolism-related indicators ALP, TRAP, OCIF, and ODF were significantly higher in OF patients than in the control group. Interestingly, the serum level of OCIF was lower in OF patients than in the control group.

Conclusion: Our study provides important evidence for the involvement of the circHIPK3/miR-378a-3p/HDAC4 pathway in the pathogenesis of OFs. The upregulation of circHIPK3 and HDAC4 and downregulation of miR-378a-3p observed in OF patients suggests their potential regulatory effects on bone metabolism. Meanwhile, abnormal expression of serum bone metabolism-related indicators may contribute to the development of OFs by disrupting the balance of bone remodeling.

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期刊介绍: Journal of Orthopaedic Surgery is an open access peer-reviewed journal publishing original reviews and research articles on all aspects of orthopaedic surgery. It is the official journal of the Asia Pacific Orthopaedic Association. The journal welcomes and will publish materials of a diverse nature, from basic science research to clinical trials and surgical techniques. The journal encourages contributions from all parts of the world, but special emphasis is given to research of particular relevance to the Asia Pacific region.
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