Lei Sun, Hao Guo, Xiaoqin Wei, Zongyan Lu, Mingyue Wang, Zeyun Yang, Yingfei Hui, Zhiguo Yu, Wu Yang
{"title":"高结晶聚酰亚胺共价有机框架作为超灵敏检测DNA嘌呤碱基的电化学传感平台。","authors":"Lei Sun, Hao Guo, Xiaoqin Wei, Zongyan Lu, Mingyue Wang, Zeyun Yang, Yingfei Hui, Zhiguo Yu, Wu Yang","doi":"10.1016/j.talanta.2023.125493","DOIUrl":null,"url":null,"abstract":"<div><p><span>It is of great significance to propose simple methods to detect DNA<span> bases sensitively for biological analysis and medical diagnosis. Herein, a highly crystalline polyimide covalent organic framework (TAPM-COF) has been successfully synthesized via a solvothermal route using pyromellitic dianhydride (PMDA) and tris(4-aminophenyl) amine (TAPA), which possessed large specific surface area (2286 m</span></span><sup>2</sup> g<sup>−1</sup><span>) and excellent thermal stability. Intriguingly, the crystallinity<span><span> of the TAPM-COF improved significantly with the increase of water content in the reaction medium. To verify this phenomenon, we synthesized TPPM-COF with two pores by pyromellitic dianhydride (PMDA) and N,N,N′,N'-tetrakis(4-aminophenyl)-1,4-benzenediamine (TPDA), which bonding was similar to TAPM-COF. Furthermore, the prepared TAPM–COF–0.3 was used to construct a novel and independent electrochemical biosensor on glassy carbon electrode for simultaneously determination of adenine<span> (A) and guanine (G) without other additives. However, to further improve signal of TAPM-COF in electrochemical sensing, the crystalline TAPM–COF–0.3 can be readily integrated with amino-functionalized </span></span>multiwalled carbon nanotubes (NH</span></span><sub>2</sub>-MWCNT) to form core-shell TAPM–COF–0.3@NH<sub>2</sub><span>-MWCNT driven by a π–π stacking interaction for more sensitive electrochemical sensing toward purine bases. In comparison to TAPM-COF/GCE, the TAPM-COF@NH</span><sub>2</sub>-MWCNT/GCE exhibited more favorable linear range and lower limit of detection. The work provided a new strategy for amplifying signal of COF in the field of electrochemical sensors.</p></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Highly crystalline polyimide covalent organic framework as electrochemical sensing platform for the ultrasensitive detection of purine bases in DNA\",\"authors\":\"Lei Sun, Hao Guo, Xiaoqin Wei, Zongyan Lu, Mingyue Wang, Zeyun Yang, Yingfei Hui, Zhiguo Yu, Wu Yang\",\"doi\":\"10.1016/j.talanta.2023.125493\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>It is of great significance to propose simple methods to detect DNA<span> bases sensitively for biological analysis and medical diagnosis. Herein, a highly crystalline polyimide covalent organic framework (TAPM-COF) has been successfully synthesized via a solvothermal route using pyromellitic dianhydride (PMDA) and tris(4-aminophenyl) amine (TAPA), which possessed large specific surface area (2286 m</span></span><sup>2</sup> g<sup>−1</sup><span>) and excellent thermal stability. Intriguingly, the crystallinity<span><span> of the TAPM-COF improved significantly with the increase of water content in the reaction medium. To verify this phenomenon, we synthesized TPPM-COF with two pores by pyromellitic dianhydride (PMDA) and N,N,N′,N'-tetrakis(4-aminophenyl)-1,4-benzenediamine (TPDA), which bonding was similar to TAPM-COF. Furthermore, the prepared TAPM–COF–0.3 was used to construct a novel and independent electrochemical biosensor on glassy carbon electrode for simultaneously determination of adenine<span> (A) and guanine (G) without other additives. However, to further improve signal of TAPM-COF in electrochemical sensing, the crystalline TAPM–COF–0.3 can be readily integrated with amino-functionalized </span></span>multiwalled carbon nanotubes (NH</span></span><sub>2</sub>-MWCNT) to form core-shell TAPM–COF–0.3@NH<sub>2</sub><span>-MWCNT driven by a π–π stacking interaction for more sensitive electrochemical sensing toward purine bases. In comparison to TAPM-COF/GCE, the TAPM-COF@NH</span><sub>2</sub>-MWCNT/GCE exhibited more favorable linear range and lower limit of detection. The work provided a new strategy for amplifying signal of COF in the field of electrochemical sensors.</p></div>\",\"PeriodicalId\":435,\"journal\":{\"name\":\"Talanta\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2023-11-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Talanta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0039914023012444\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0039914023012444","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Highly crystalline polyimide covalent organic framework as electrochemical sensing platform for the ultrasensitive detection of purine bases in DNA
It is of great significance to propose simple methods to detect DNA bases sensitively for biological analysis and medical diagnosis. Herein, a highly crystalline polyimide covalent organic framework (TAPM-COF) has been successfully synthesized via a solvothermal route using pyromellitic dianhydride (PMDA) and tris(4-aminophenyl) amine (TAPA), which possessed large specific surface area (2286 m2 g−1) and excellent thermal stability. Intriguingly, the crystallinity of the TAPM-COF improved significantly with the increase of water content in the reaction medium. To verify this phenomenon, we synthesized TPPM-COF with two pores by pyromellitic dianhydride (PMDA) and N,N,N′,N'-tetrakis(4-aminophenyl)-1,4-benzenediamine (TPDA), which bonding was similar to TAPM-COF. Furthermore, the prepared TAPM–COF–0.3 was used to construct a novel and independent electrochemical biosensor on glassy carbon electrode for simultaneously determination of adenine (A) and guanine (G) without other additives. However, to further improve signal of TAPM-COF in electrochemical sensing, the crystalline TAPM–COF–0.3 can be readily integrated with amino-functionalized multiwalled carbon nanotubes (NH2-MWCNT) to form core-shell TAPM–COF–0.3@NH2-MWCNT driven by a π–π stacking interaction for more sensitive electrochemical sensing toward purine bases. In comparison to TAPM-COF/GCE, the TAPM-COF@NH2-MWCNT/GCE exhibited more favorable linear range and lower limit of detection. The work provided a new strategy for amplifying signal of COF in the field of electrochemical sensors.
期刊介绍:
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.