CRISPR 技术在基因组工程方面的最新进展。

IF 2.9 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY BMB Reports Pub Date : 2024-01-01

Youngsik Lee, Yeounsun Oh, Seung Hwan Lee

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引用次数: 0

摘要

随着 CRISPR 技术的发展，有效编辑靶基因的时代已经到来。然而，由于CRISPR元件的固有特性，在识别靶DNA时出现的脱靶问题仍是未来需要攻克的最大难题。本综述从CRISPR的结构方面分析了诱导这种非预期脱靶编辑的原理，并总结了迄今为止为减少脱靶编辑而开发的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Recent advances in genome engineering by CRISPR technology.

Due to the development of CRISPR technology, the era of effective editing of target genes has arrived. However, the offtarget problem that occurs when recognizing target DNA due to the inherent nature of CRISPR components remains the biggest task to be overcome in the future. In this review, the principle of inducing such unintended off-target editing is analyzed from the structural aspect of CRISPR, and the methodology that has been developed to reduce off-target editing until now is summarized. [BMB Reports 2024; 57(1): 12-18].

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来源期刊

BMB Reports 生物-生化与分子生物学

CiteScore

5.10

自引率

7.90%

发文量

141

审稿时长

1 months

期刊介绍： The BMB Reports (BMB Rep, established in 1968) is published at the end of every month by Korean Society for Biochemistry and Molecular Biology. Copyright is reserved by the Society. The journal publishes short articles and mini reviews. We expect that the BMB Reports will deliver the new scientific findings and knowledge to our readers in fast and timely manner.