氟苯尼考引起的肠道菌群失调会增加斑马鱼在建议停药期后感染嗜水气单胞菌的易感性

IF 1.5 4区 农林科学 Q3 FISHERIES Journal of aquatic animal health Pub Date : 2023-12-07 DOI:10.1002/aah.10211
Francisca A. Burgos, Wenlong Cai, Covadonga R. Arias
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引用次数: 0

摘要

氟苯尼考是美国食品和药物管理局(FDA)批准的一种广谱抗生素,用于治疗食用鱼的全身和体外细菌感染。本研究的目的是评估氟苯尼考(FFC)药用饲料对斑马鱼肠道微生物群的影响,以确定 i) 氟苯尼考药用饲料的治疗剂量是否会诱发菌群失调,以及 ii) 肠道微生物群发生变化的鱼类是否更容易受到常见机会性鱼类病原体嗜水气单胞菌的后续感染。我们使用 16S rRNA 基因测序分析了使用氟化膳食饲料的斑马鱼的肠道微生物群,结果发现氟化膳食饲料诱导了肠道微生物群的紊乱。在所有处理组中都观察到了菌群失调,细菌多样性显著增加,其特点是蛋白菌明显增多,支原体和鲸杆菌在处理动物中急剧减少,但没有明显的临床症状或死亡。此外,在建议的 15 天抗生素停药期过后,变形菌的增加并没有明显减少,而且与普通饲料组相比,使用 FFC 药物饲料的斑马鱼在随后受到嗜水甲藻挑战时的死亡率明显更高。有趣的是,肠道微生物组组成的最显著变化发生在药物治疗后期与停药期开始之间的过渡时期,而不是在感染嗜水气单胞菌期间。总之,按建议剂量投喂含氟全氟鱼饲料会诱发斑马鱼肠道菌群失调,而且在建议的停药期后也不会恢复到基线水平。我们的研究结果表明,在鱼类中使用抗生素会引起与之前在哺乳动物中描述的类似的反应,并可能使宿主更容易受到机会性病原体的后续感染。这项使用受控模型系统进行的研究表明,水产养殖中的抗生素可能会对鱼类的整体健康产生长期影响。
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Gut dysbiosis induced by florfenicol increases susceptibility to Aeromonas hydrophila infection in Zebrafish Danio rerio after the recommended withdrawal period

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来源期刊
Journal of aquatic animal health
Journal of aquatic animal health 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
25
审稿时长
>24 weeks
期刊介绍: The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.
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