通过细胞和遗传生物标记评估纠正动物体力衰竭状态的方法

В.Н. Каркищенко, И.А. Помыткин, Н.В. Петрова, И.А. Васильева, О.В. Алимкина, Н.В. Станкова, Н.А. Ларюшина, V. Karkischenko, I. A. Pomytkin, N. V. Petrova, I. A. Vasil’eva, Oksana V. Alimkina, Nataliia V. Stankova, N. A. Laryushina
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引用次数: 0

摘要

在这项工作中,我们研究了一些药物制剂对基因表达的影响,这些基因被用作迷你猪在虚弱的体育锻炼条件下的分子靶标。在进行的实验中,比较了以下制剂的有效性:“Mio-Activ-Sport”膳食补充剂,鹿麝香提取物脂质体,鼻内胰岛素和人参脂质体制剂。选择的生物标志物包括白细胞血分数和淋巴细胞中NFE2L2和HMGB1基因的mRNA表达。其中,麝香脂质体提取物的效果最好。因此,在7天的治疗中,这种制剂几乎完全阻止了身体负荷的促炎作用。同时,脂质体麝麝香提取物导致负责机体抗氧化防御的NFE2L2基因表达增加数倍。在作用方面,脂质体麝麝香提取物优于参考药物胰岛素,其保护作用从科学文献中众所周知。这些发现证实了麝香制剂在医学康复中的应用前景。
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Evaluation of Approaches for Correcting the State of Animals under Debilitating Physical Exertion by Cellular and Genetic Biomarkers
In this work, we investigate the effect of a number of pharmaceutical preparations on the expression of genes, which are used as molecular targets under the conditions of debilitating physical exercise in minipigs. In the conducted experiment, the effectiveness of the following preparations was compared: “Mio-Activ-Sport” dietary supplement, a liposomal deer musk extract, intranasal insulin, and a liposomal ginseng preparation. The selected biomarkers included the leukocyte blood fraction and mRNA expression of NFE2L2 and HMGB1 genes in lymphocytes. Among the studied preparations, the liposomal deer musk extract showed the highest effectiveness. Thus, during seven days of therapy, this preparation almost completely prevented the pro-inflammatory effect of physical load. At the same time, the liposomal deer musk extract led to a manyfold increase in the expression of NFE2L2 gene, which is responsible for antioxidant defense of the organism. In terms of action, the liposomal deer musk extract outperformed insulin – the reference drug, whose protective effects are well known from the scientific literature. These findings confirm the prospects of deer musk preparations for medical rehabilitation.
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