Pachyrhizus erosus (L.) Urb.通过 PPAR 通路在 MDI 诱导的 3T3-L1 细胞系中的抗肥胖作用

IF 3.4 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Cosmetics Pub Date : 2023-12-01 DOI:10.3390/cosmetics10060164
Moon-Hee Choi, Seung-Hwa Yang, Yeo-Jin Lee, Jeong Ho Sohn, Ki Sun Lee, H. Shin
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引用次数: 0

摘要

厚根菌(L.)市区。是一种热带多年生藤本植物,原产于墨西哥南部、东南亚、中美洲和非洲。在这项研究中,我们分析和鉴定了不同的多酚类化合物和异黄酮,并研究了它们作为天然保健食品资源的潜在抗肥胖活性。提取过程包括干燥山药豆,然后用70%乙醇提取,蒸发和冷冻干燥。采用正己烷、乙酸乙酯(EtOAc)、丁醇(BuOH)和水进行分层分离。EtOAc部位对ABTS自由基的IC50值为531.77µg/mL,在各实验组中表现出最高的抗氧化活性。α-葡萄糖苷酶和脂肪酶抑制实验的IC50值分别为873.07µg/mL和915.02µg/mL。采用高效液相色谱法(HPLC)和液相色谱-质谱联用技术(LC-MS/MS)对黄花参根提取物中的异黄酮成分进行了检测,鉴定出其中含有大豆苷元、染料木素和鱼藤酮。黄豆根提取物中含量最高的异黄酮是大豆黄酮。为了验证EtOAc部分和大豆苷元的抗肥胖活性,我们使用MDI(3-异丁基-1-甲基黄嘌呤,地米米松,胰岛素)处理3T3-L1前脂肪细胞8天。油红O染色实验表明,乙酸乙酯部分和大豆苷元处理组的脂质含量呈浓度依赖性降低。此外,我们检测了瘦素- ppar - fas通路相关蛋白的表达模式,揭示了肥胖相关蛋白的浓度依赖性减少。
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Anti-Obesity Effect of Daidzein Derived from Pachyrhizus erosus (L.) Urb. Extract via PPAR Pathway in MDI-Induced 3T3-L1 Cell Line
Pachyrhizus erosus (L.) Urb. is a tropical perennial vine plant native to southern Mexico, Southeast Asia, Central America, and Africa. In this study, we analyzed and identified various polyphenolic compounds and isoflavones present in ethanolic P. erosus root extracts and investigated their potential anti-obesity activity as a natural health food resource. The extraction process involved drying the yam bean, followed by extraction with 70% ethanol, evaporation, and freeze-drying. Fractionation was achieved through layer separation using n-hexane, ethyl acetate (EtOAc), butanol (BuOH), and water. The EtOAc fraction exhibited the highest antioxidant activity among the experimental groups, with an IC50 value of 531.77 µg/mL for ABTS radical scavenging. In α-glucosidase and lipase inhibition assays, IC50 values were determined to be 873.07 µg/mL and 915.02 µg/mL, respectively. Using HPLC and LC-MS/MS, we detected isoflavone components in P. erosus root extracts, identifying daidzein, genistein, and rotenone among them. Daidzein was the most abundant isoflavone in P. erosus root extracts. To validate the anti-obesity activity in the EtOAc fraction and daidzein, we used 3T3-L1 preadipocytes treated with MDI (3-isobutyl-1-methylxanthine, dexamethasone, insulin) for 8 days. Oil Red O staining experiments demonstrated a concentration-dependent reduction in lipid content in the EtOAc fraction and daidzein treatment groups. Additionally, we examined the expression pattern of proteins related to the leptin-PPAR-FAS Pathway, revealing a concentration-dependent decrease in obesity-related proteins.
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来源期刊
Cosmetics
Cosmetics Medicine-Surgery
CiteScore
5.20
自引率
12.10%
发文量
108
审稿时长
8 weeks
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