Cichorium intybus L. Distillate 对雄性小鼠生殖参数的改善作用

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL Iranian Journal of Medical Sciences Pub Date : 2023-11-01 DOI:10.30476/IJMS.2023.96653.2826
Shima Fakher, Saeid Karbalay-Doust, Ali Noorafshan, Atefeh Seghatoleslam
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引用次数: 0

摘要

背景:Cichorium intybus L. (Kasni)蒸馏物在东方国家被广泛用作提高男性生育能力的安全草药饮品。然而,对生育力参数的潜在影响和可能的不良反应尚未进行实验研究。本研究旨在评估 Cichorium intybus L. 蒸馏液(CD)对雄性小鼠生育能力的影响:在本研究中(伊朗设拉子),30 只雄性小鼠(30-35 克)被分为三组。10 只小鼠接受蒸馏水(DW)治疗 5 周,作为对照组。另外 10 只小鼠(CD1/2 组)接受菊苣蒸馏水 1/2 稀释液,另外 10 只小鼠接受菊苣蒸馏水 CD1/4 稀释液(CD1/4 组),自由饮用 3 周,之后接受蒸馏水 2 周。实验小鼠在第 35 天被处死,并进行精子分析和血清采集,以进一步检测 FSH、LH、睾酮和一些肝肾功能指标。我们使用左侧睾丸进行立体分析,切除右侧睾丸以研究雄激素受体基因的表达。统计分析采用 SPSS 18.0,均值±SD 值采用单因素方差分析(ANOVA),组间比较采用 Dunnett 后分析。在立体学研究中,采用 Kruskal-Wallis 方法进行成对比较,以比较各组。结果的 P 值被认为具有统计学意义:与对照组相比,CD1/2治疗可使血清FSH(P=0.002)、LH(P=0.009)、睾酮(P=0.034)、曲细精管上皮细胞体积(P=0.029)和长度(P=0.028)以及Leydig细胞数量(P=0.009)升高。与对照组相比,服用CD1/2(P=0.038)和CD1/4(P=0.013)可显著增加精子数量:结果表明,使用菊苣蒸馏物可以改善雄性小鼠的激素水平和精子数量。
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The Ameliorative Impact of Cichorium intybus L. Distillate on Reproductive Parameters in Male Mice.

Background: Cichorium intybus L. (Kasni) distillate is widely used in Eastern countries as a safe herbal drink to improve male fertility. However, the potential effects on fertility parameters and possible adverse effects have not been studied experimentally. The current study aims to evaluate the impact of Cichorium intybus L. distillate (CD) on male mice fertility.

Methods: In the present study (Shiraz, Iran), 30 male mice (30-35 g) were divided into three groups. 10 mice received distilled water (DW) for five weeks as the control group. Another 10 mice, named group CD1/2, received chicory distillate of 1/2 dilution, and the other 10 mice received chicory distillate of CD1/4 dilution as CD1/4 group, ad libitum for three weeks, and they received DW for two weeks afterward. Experimental mice were sacrificed on day 35, and sperm analysis and sera collection were performed for further investigation of FSH, LH, testosterone, and some liver and kidney function parameters. We used the left testis for stereological analysis, and the right one was excised to investigate the expression of the androgen receptor gene. For statistical analysis using SPSS 18.0, mean±SD values were analyzed by one-way analysis of variance (ANOVA) with Dunnett's analysis as post hoc to compare between groups. In stereological investigations, the Kruskal-Wallis method was used for pairwise comparisons to compare groups. The P value was considered statistically significant at P<0.05.

Results: Treatment with CD1/2 resulted in the elevation of serum FSH (P=0.002), LH (P=0.009), testosterone (P=0.034), seminiferous tubule epithelium volume (P=0.029) and length (P=0.028), and Leydig cells number (P=0.009) in comparison with the control group. Administrating CD1/2 (P=0.038) and CD1/4 (P=0.013) significantly increased sperm count compared to the control group.

Conclusion: The results revealed that using chicory distillate can improve hormone levels and sperm count in male mice.

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来源期刊
Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
0.00%
发文量
84
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of com­muni­cation for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science re­search experiences on prevalent diseases in the region and analysis of various regional problems.
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