利用聚乙二醇对多个番茄品种进行耐旱性筛选

S. Avivi, Novrida Yanti Sitompul, T. A. Siswoyo, Mohammad Ubaidillah
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引用次数: 0

摘要

长期干旱季节会造成干旱胁迫,对番茄植株的生长产生不利影响,导致作物歉收。因此,需要研究番茄品种对干旱胁迫的抗性。番茄抗旱性筛选的推荐技术是使用聚乙二醇(PEG)进行离体培养。研究的目的是获得新品种,作为抗旱品种的希望品系。研究方法包括使用 5 个番茄品种(Ratna、Intan、Chung、Palupi 和 Amelia)和 5 种浓度的 PEG 化学品(0%、5%、10%、15% 和 20%)。使用次氯酸盐灭菌的每个番茄品种的每粒种子都在 MS0 培养基上发芽,然后将大小为 ± 0.3 厘米的发芽种子转移到各处理浓度的 MS0 + PEG 处理培养基上。然后观察在 PEG 处理培养基上种植的种子的生长情况(± 4 周)。研究数据采用方差分析(ANOVA)进行分析,并在 5%的显著性水平下采用 DMRT 进行分析。结果表明,Palupi 品种对干旱胁迫最耐受,而 Ratna 品种对干旱胁迫最不耐受。使用的 PEG 浓度越高,对株高、根长和根鲜重的抑制作用越大。
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Drought tolerance selection of several tomato varieties by using polyethylene glycol
A prolonged dry season can cause drought stress and have an unfavorable impact on the growth of tomato plants, resulting in crop failure. Therefore, research is needed on the resistance of tomato varieties to drought stress. The recommended technique for drought resistance screening in tomatoes is in vitro cultivation using Polyethylene Glycol (PEG). The research aimed to obtain new varieties that could be used as promising lines for varieties tolerant to drought stress. The research method used included using ingredients in the form of 5 varieties of tomatoes (Ratna, Intan, Chung, Palupi, and Amelia) and 5 concentrations of PEG chemicals (0%, 5%, 10%, 15%, and 20%). Each seed of each tomato variety that had been sterilized using hypochlorite was germinated on MS0 media, then the germinated seeds with a size of ± 0.3 cm were transferred to MS0 + PEG treatment medium with each treatment concentration. The seeds that had been planted on the PEG-treated medium were then observed for growth for ± 4 weeks. The research data were analyzed using analysis of variance (ANOVA) and followed with DMRT at the 5% significance level. Based on the results, Palupi variety is the most tolerant variety, and Ratna variety is the most intolerant variety to drought stress. The higher the concentration of PEG used, the more it inhibits plant height, root length, and fresh weight of the roots.
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审稿时长
10 weeks
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