预测宫颈癌患者放疗后早期死亡的外泌体非编码 RNA 和血象图

IF 4.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY International Journal of Molecular Sciences Pub Date : 2023-12-21 DOI:10.3390/ijms25010126
O. Cho
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引用次数: 0

摘要

同期放化疗(CCRT)与各种癌症的疾病进展加速和早期死亡(ED)有关。本研究旨在评估接受CCRT治疗的宫颈癌患者血浆中外泌体非编码核糖核酸(RNA)(ncRNA)水平和血细胞动态与ED预测的相关性。通过倾向得分匹配,对 370 名接受 CCRT 治疗的患者的全血细胞计数(CBC)进行了比较。42 名患者(队列 2)的 84 份样本在 CCRT 治疗前后的 ncRNA 和信使 RNA (mRNA) 表达差异以对数折叠变化(log2FC)表示。通过构建网络将 CBC 与 ED 表达相关的 RNA 联系起来。通过对网络中的所有 RNA 组合进行多元回归,从中筛选出关键 RNA,并利用富集分析对与 CBC 动态相关的 ncRNA 进行功能表征。队列 1(120 名患者)显示出中性粒细胞绝对计数(ANC)升高与 ED 之间的相关性。队列 2 显示,microRNA (miR)-574-3p 和长基因间非蛋白编码 (LINC)01003 ncRNA 的流行,其表达分别与 ANC 和血红蛋白值相关。相反,酰基辅酶 A 硫代酯酶 9 (ACOT9) mRNA 与所有 CBC 成分相关。对CCRT后ncRNA水平和CBC值的综合分析表明,miR-574-3p-LINC01003-ACOT9 log2FC)< 0的患者30个月的疾病特异性生存率更高。这些发现表明,miR-574-3p 和 LINC01003 可作为 ED 的预后生物标志物。
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Post-Radiotherapy Exosomal Non-Coding RNA and Hemograms for Early Death Prediction in Patients with Cervical Cancer
Concurrent chemo-radiotherapy (CCRT) is linked with accelerated disease progression and early death (ED) in various cancers. This study aimed to assess the association of plasma levels of exosomal non-coding ribonucleic acid (RNA) (ncRNA) and blood cell dynamics with ED prediction in patients with cervical cancer undergoing CCRT. Using propensity score matching, a comparison of complete blood counts (CBCs) was performed among 370 CCRT-treated patients. Differences in ncRNA and messenger RNA (mRNA) expression before and after CCRT in 84 samples from 42 patients (cohort 2) were represented as logarithmic fold change (log2FC). Networks were constructed to link the CBCs to the RNAs whose expression correlated with ED. From the key RNAs selected using multiple regression of all RNA combinations in the network, CBC dynamics-associated ncRNAs were functionally characterized using an enrichment analysis. Cohort 1 (120 patients) exhibited a correlation between elevated absolute neutrophil counts (ANC) and ED. Cohort 2 exhibited a prevalence of microRNA (miR)-574-3p and long intergenic non-protein coding (LINC)01003 ncRNA, whose expression correlated with ANC and hemoglobin values, respectively. Conversely, acyl-coenzyme A thioesterase 9 (ACOT9) mRNA was relevant to all CBC components. An integrative analysis of post-CCRT ncRNA levels and CBC values revealed that the patients with miR-574-3p-LINC01003-ACOT9 log2FC) < 0 had a better prospect of 30-month disease-specific survival. These findings indicate that miR-574-3p and LINC01003 could serve as ED prognostic biomarkers.
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来源期刊
International Journal of Molecular Sciences
International Journal of Molecular Sciences Chemistry-Organic Chemistry
CiteScore
8.10
自引率
10.70%
发文量
13472
审稿时长
17.49 days
期刊介绍: The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).
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