D. Valić, Matej Kristan Mirković, V. Besendorfer, E. Teskeredžić
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引用次数: 0
摘要
保护本土物种,尤其是特有物种至关重要。通常,仅从形态上确定物种是不够的;因此,我们对克尔卡河中的伊利里亚鲢鱼(Squalius illyricus)和兹尔曼亚鲢鱼(Squalius zrmanjae)进行了分子系统学分析。为了确定线粒体基因细胞色素 b 和非编码核区 Cyfun P 的遗传特征,对每个物种的 15 个标本进行了分析。获得的序列与 GenBank 中的相似序列进行了比对,以确定这些物种的分类和系统发育位置。获得的分子结果表明,克尔卡河的 S. zrmanjae 的核区与达尔马提亚白鲑(Scardinius dergle)相似。这一结果意味着这两个种属之间存在引种事件和遗传信息的转移。所调查的物种已被列入《世界自然保护联盟濒危物种红色名录》,其生物数据稀缺,需要进一步调查和保护。
Molecular Analysis of Two Endemic Squalius Species: Evidence for Intergeneric Introgression among Cyprinids and Conservation Issues
Conservation of indigenous species, especially endemic ones, is of the utmost importance. Morphological determination of species is usually not sufficient; therefore, molecular phylogenetic analyses of the Illyrian chub, Squalius illyricus, and the Zrmanja chub, Squalius zrmanjae, from the Krka River were performed. For the genetic characterization of the mitochondrial gene cytochrome b and the non-coding nuclear region Cyfun P, 15 specimens from each species were subjected to analysis. The obtained sequences were aligned with similar ones from GenBank to determine the taxonomic and phylogenetic position of these species. The obtained molecular results imply that S. zrmanjae from the Krka River has a nuclear region that resembles Dalmatian rudd, Scardinius dergle. This result implies an introgression event and the transfer of genetic information between the two genera. The investigated species are on the IUCN Red List of Threatened Species, their biological data are scarce, and further investigation and protection are needed.