从尼日利亚伊莫州奥基圭牧民那里获得的生牛乳的细菌学质量

C. Uzoh, C. O. Nworie, E. E. Eze, P. Igwe, C. Ugwuocha, K. Aroh, I. O. Abanukam, N. Onuoha, E. C. Okafor
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摘要

本研究旨在检测从伊莫州奥基圭牧民那里获得的生牛奶的细菌学质量。研究采用了标准微生物学程序。总共从奥基圭市的不同街道采集了五份样本,装在无菌样本容器中。对每份样本进行 10 倍序列稀释,第 4 管至第 6 管分别接种在营养琼脂、沙门氏菌-志贺氏菌琼脂和麦康凯琼脂上,采用涂布平板法,在 37oC 温度下培养 24 小时。经鉴定,微生物包括金黄色葡萄球菌、科里奈杆菌、伤寒沙门氏菌、链球菌、大肠杆菌、乳酸杆菌和伤寒沙门氏菌。生牛乳的总存活数介于 6.6 x105CFU/ml 至 1.01 x106CFU/ml 之间,而总大肠菌群数介于 6.2x106 CFU/ml 至 1.7x106 CFU/ml 之间。发现生牛奶中含有一些病原微生物,摄入后可导致感染。
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The Bacteriological Quality of Raw Cow Milk Obtained from Herdsmen in Okigwe, Imo State, Nigeria
This study was conducted to examine the bacteriological quality of cow’s raw milk obtained from herdsmen at Okigwe, Imo States. Standard microbiological procedure was used to carry out this research. A total number of five samples were gotten from different streets in Okigwe metropolis in sterile specimen containers. Ten fold serial dilution was carried with each of the sample with the 4th tube to 6th tube being inoculated on Nutrient agar, Salmonella- Shigella agar and MacConkey agar by spread plate method and incubated at 37oC for 24 hours. The microorganisms were identified were Staphylococcus aureus, Corynebacterium sp, Salmonella typhi, Streptococcus sp, E. coli, Lactobacillus sp and Salmonella typhi. The total viable count for raw cow milk ranged from 6.6 x105CFU/ml to 1.01 x 106CFU/ml while total coliform count was from 6.2x106 CFU/ml to 1.7x106 CFU/ml. They frequency of occurrence of Lactobacillus sp was highest with a value 85 and the lowest frequency was Salmonella typhi II with a value of 2. The raw cow milk was observed to harbor some pathogenic microorganisms which can cause infections when ingested. 
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