通过整合单细胞和空间转录组学发现肠道巨噬细胞

IF 5 3区 医学 Q1 GENETICS & HEREDITY Genes and immunity Pub Date : 2023-12-23 DOI:10.1038/s41435-023-00242-7
Alba Garrido-Trigo, Marisol Veny, Azucena Salas
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引用次数: 0

摘要

单细胞 RNA 测序(scRNA-seq)扩大了我们对组织细胞组成的了解,并为生物医学领域提供了一种宝贵的工具,能以以前无法达到的分辨率绘制与疾病相关的模式图[1]。此外,单细胞转录组学还能发现细胞的动态和过渡状态,对传统的离散细胞群概念提出了挑战。将单细胞转录组学应用于组织分析的启示之一是,细胞系在健康和疾病中都存在一定程度的异质性。然而,scRNA-seq 也带来了一些挑战,包括在同一组织内和不同组织间的细胞类型注释难以达成共识,这可能会限制交叉研究的验证。此外,scRNA-seq 数据的解读也有局限性;虽然细胞的转录谱反映了细胞的系谱和状态,但可能不足以推断其在特定组织或疾病环境中的功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Uncovering intestinal macrophages through the integration of single-cell and spatial transcriptomics
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来源期刊
Genes and immunity
Genes and immunity 医学-免疫学
CiteScore
8.90
自引率
4.00%
发文量
28
审稿时长
6-12 weeks
期刊介绍: Genes & Immunity emphasizes studies investigating how genetic, genomic and functional variations affect immune cells and the immune system, and associated processes in the regulation of health and disease. It further highlights articles on the transcriptional and posttranslational control of gene products involved in signaling pathways regulating immune cells, and protective and destructive immune responses.
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