{"title":"描述从制药厂生产车间空气中分离出的放线菌 Nesterenkonia aerolata sp.","authors":"Ming-Hui Song, Wen-Long Ma, Ning Zhang, Wei He, Hong-Chuan Wang, Shuang Wang, Yi-Ling Fan, Dao-Feng Zhang","doi":"10.1007/s10482-023-01905-1","DOIUrl":null,"url":null,"abstract":"<div><p>During our studies on the microorganism diversity from air of manufacturing shop in a pharmaceutical factory in Shandong province, China, a Gram-stain-positive, aerobic, cocci-shaped bacterium, designated LY-0111<sup>T</sup>, was isolated from a settling dish<i>.</i> Strain LY-0111<sup>T</sup> grew at temperature of 10–42 °C (optimum 35 °C), pH of 5.0–10.0 (optimum pH 7.0) and NaCl concentration of 1–12% (optimum 0.5–3%, w/v). Based on the 16S rRNA gene sequence analysis, the strain shared the highest sequence similarities to <i>Nesterenkonia halophila</i> YIM 70179<sup>T</sup> (96.2%), and was placed within the radiation of <i>Nesterenkonia</i> species in the phylogenetic trees. The genome of the isolate was sequenced, which comprised 2,931,270 bp with G + C content of 66.5%. A supermatrix tree based on the gene set bac120 indicated that LY-0111<sup>T</sup> was close related to <i>Nesterenkonia xinjiangensis</i> YIM 70097<sup>T</sup> (16S rRNA gene sequence similarity 95.3%). Chemotaxonomic analysis indicated that the main respiratory quinones were MK-7, MK-8, and MK-9, the predominant cellular fatty acids were anteiso-C<sub>15:0</sub> and iso-C<sub>15:0</sub>, and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. According to the phenotypic, chemotaxonomic and phylogenetic features, strain LY-0111<sup>T</sup> is considered to represent a novel species, for which the name <i>Nesterenkonia aerolata</i> sp. nov. is proposed. The type strain is LY-0111<sup>T</sup> (= JCM 36375<sup>T</sup> = GDMCC 1.3945<sup>T</sup>). In addition, <i>Nesterenkonia jeotgali</i> was proposed as a later synonym of <i>Nesterenkonia sandarakina</i>, according to the ANI (96.8%) and dDDH (72.9%) analysis between them.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Description of Nesterenkonia aerolata sp. nov., an actinobacterium isolated from air of manufacturing shop in a pharmaceutical factory\",\"authors\":\"Ming-Hui Song, Wen-Long Ma, Ning Zhang, Wei He, Hong-Chuan Wang, Shuang Wang, Yi-Ling Fan, Dao-Feng Zhang\",\"doi\":\"10.1007/s10482-023-01905-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>During our studies on the microorganism diversity from air of manufacturing shop in a pharmaceutical factory in Shandong province, China, a Gram-stain-positive, aerobic, cocci-shaped bacterium, designated LY-0111<sup>T</sup>, was isolated from a settling dish<i>.</i> Strain LY-0111<sup>T</sup> grew at temperature of 10–42 °C (optimum 35 °C), pH of 5.0–10.0 (optimum pH 7.0) and NaCl concentration of 1–12% (optimum 0.5–3%, w/v). Based on the 16S rRNA gene sequence analysis, the strain shared the highest sequence similarities to <i>Nesterenkonia halophila</i> YIM 70179<sup>T</sup> (96.2%), and was placed within the radiation of <i>Nesterenkonia</i> species in the phylogenetic trees. The genome of the isolate was sequenced, which comprised 2,931,270 bp with G + C content of 66.5%. A supermatrix tree based on the gene set bac120 indicated that LY-0111<sup>T</sup> was close related to <i>Nesterenkonia xinjiangensis</i> YIM 70097<sup>T</sup> (16S rRNA gene sequence similarity 95.3%). Chemotaxonomic analysis indicated that the main respiratory quinones were MK-7, MK-8, and MK-9, the predominant cellular fatty acids were anteiso-C<sub>15:0</sub> and iso-C<sub>15:0</sub>, and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. According to the phenotypic, chemotaxonomic and phylogenetic features, strain LY-0111<sup>T</sup> is considered to represent a novel species, for which the name <i>Nesterenkonia aerolata</i> sp. nov. is proposed. The type strain is LY-0111<sup>T</sup> (= JCM 36375<sup>T</sup> = GDMCC 1.3945<sup>T</sup>). In addition, <i>Nesterenkonia jeotgali</i> was proposed as a later synonym of <i>Nesterenkonia sandarakina</i>, according to the ANI (96.8%) and dDDH (72.9%) analysis between them.</p></div>\",\"PeriodicalId\":50746,\"journal\":{\"name\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-01-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10482-023-01905-1\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10482-023-01905-1","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Description of Nesterenkonia aerolata sp. nov., an actinobacterium isolated from air of manufacturing shop in a pharmaceutical factory
During our studies on the microorganism diversity from air of manufacturing shop in a pharmaceutical factory in Shandong province, China, a Gram-stain-positive, aerobic, cocci-shaped bacterium, designated LY-0111T, was isolated from a settling dish. Strain LY-0111T grew at temperature of 10–42 °C (optimum 35 °C), pH of 5.0–10.0 (optimum pH 7.0) and NaCl concentration of 1–12% (optimum 0.5–3%, w/v). Based on the 16S rRNA gene sequence analysis, the strain shared the highest sequence similarities to Nesterenkonia halophila YIM 70179T (96.2%), and was placed within the radiation of Nesterenkonia species in the phylogenetic trees. The genome of the isolate was sequenced, which comprised 2,931,270 bp with G + C content of 66.5%. A supermatrix tree based on the gene set bac120 indicated that LY-0111T was close related to Nesterenkonia xinjiangensis YIM 70097T (16S rRNA gene sequence similarity 95.3%). Chemotaxonomic analysis indicated that the main respiratory quinones were MK-7, MK-8, and MK-9, the predominant cellular fatty acids were anteiso-C15:0 and iso-C15:0, and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. According to the phenotypic, chemotaxonomic and phylogenetic features, strain LY-0111T is considered to represent a novel species, for which the name Nesterenkonia aerolata sp. nov. is proposed. The type strain is LY-0111T (= JCM 36375T = GDMCC 1.3945T). In addition, Nesterenkonia jeotgali was proposed as a later synonym of Nesterenkonia sandarakina, according to the ANI (96.8%) and dDDH (72.9%) analysis between them.
期刊介绍:
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.