图尔基耶新记录的 Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983 (Dorylaimida: Longidoridae) 的形态特征和分子分析

Serkan Çeli̇k, F. Akyazi
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摘要

研究目的本研究旨在确定首次在土耳其记录的 Longidorus moesicus 的形态、形态计量学和分子特征。 材料与方法:2017 年在土耳其亚洛瓦省的玫瑰种植温室中进行了线虫调查。采用柯布筛法和离心浮选技术从土壤样本中提取线虫。利用形态特征和形态测量来鉴定所获得的线虫。此外,还通过 28S rRNA 基因 D2/D3 扩增区序列的分子分析确认了鉴定结果。 结果针线虫属中的 Longidorus(Micoletzky,1922 年)包括许多外寄生线虫种类,它们传播肾小球病毒,并通过直接取食根细胞造成损害。在考察的土壤中发现了一种龙线虫。 结论据我们所知,这是土耳其首次报道 Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983。
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Morphological Characteristics and Molecular Analysis of Newly Recorded Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983 (Dorylaimida: Longidoridae) From Türkiye
Objective: This study was conducted to determine the morphological, morphometric, and molecular characteristics of Longidorus moesicus, which was first recorded in Türkiye. Materials and Methods: A nematode survey was conducted in rose cultivation greenhouses in 2017 in Yalova province, Türkiye. Nematodes were extracted from soil samples using Cobb's sieve method and centrifugal flotation technique. Morphological characters and morphometric measurements were used to identify the obtained nematodes. Additionally, identification was confirmed by molecular analyses of the 28S rRNA gene D2/D3 expansion region sequences. Results: Needle nematodes in the genus Longidorus (Micoletzky, 1922) include many ectoparasitic nematode species that transmit nepoviruses as well as causing damage by direct feeding on root cells. A Longidorus species was found in the examined soils. Conclusion: To our knowledge, this is the first report of Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983 in Türkiye.
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