{"title":"伊朗西北部乌尔米耶市宠物猫中五联巴顿氏菌的基因组检测和系统发育分析","authors":"Sima Alempour Rajabi , Abdolghaffar Ownagh , Mojtaba Hadian","doi":"10.1016/j.cimid.2024.102125","DOIUrl":null,"url":null,"abstract":"<div><p>The aim of this study was to investigate the presence and genetic characteristics of <span><em>Bartonella quintana</em></span><span> in pet cats from Urmia City, located in the northwest of Iran. Blood samples were collected from 200 cats, and their age, gender, and breed were noted. Nested-PCR and sequencing were used to identify </span><em>B. quintana</em> in positive samples, and the <em>ftsZ</em><span> gene sequences were analyzed using BioEdit software. The gene sequence obtained in this study exhibited 100.00 % similarity to reference sequences in the GenBank® database, and a phylogenetic tree was constructed using MEGA11. The results revealed that 15 % of the cats (30 out of 200 blood samples) tested positive for the </span><em>B. quintana</em> gene, with a 95 % confidence interval of 10.71 % to 20.61 %.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"105 ","pages":"Article 102125"},"PeriodicalIF":2.0000,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genomic detection and phylogenetic analysis of Bartonella quintana in pet cats from Urmia City, Northwest Iran\",\"authors\":\"Sima Alempour Rajabi , Abdolghaffar Ownagh , Mojtaba Hadian\",\"doi\":\"10.1016/j.cimid.2024.102125\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The aim of this study was to investigate the presence and genetic characteristics of <span><em>Bartonella quintana</em></span><span> in pet cats from Urmia City, located in the northwest of Iran. Blood samples were collected from 200 cats, and their age, gender, and breed were noted. Nested-PCR and sequencing were used to identify </span><em>B. quintana</em> in positive samples, and the <em>ftsZ</em><span> gene sequences were analyzed using BioEdit software. The gene sequence obtained in this study exhibited 100.00 % similarity to reference sequences in the GenBank® database, and a phylogenetic tree was constructed using MEGA11. The results revealed that 15 % of the cats (30 out of 200 blood samples) tested positive for the </span><em>B. quintana</em> gene, with a 95 % confidence interval of 10.71 % to 20.61 %.</p></div>\",\"PeriodicalId\":50999,\"journal\":{\"name\":\"Comparative Immunology Microbiology and Infectious Diseases\",\"volume\":\"105 \",\"pages\":\"Article 102125\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-01-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Immunology Microbiology and Infectious Diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S014795712400002X\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Immunology Microbiology and Infectious Diseases","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S014795712400002X","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Genomic detection and phylogenetic analysis of Bartonella quintana in pet cats from Urmia City, Northwest Iran
The aim of this study was to investigate the presence and genetic characteristics of Bartonella quintana in pet cats from Urmia City, located in the northwest of Iran. Blood samples were collected from 200 cats, and their age, gender, and breed were noted. Nested-PCR and sequencing were used to identify B. quintana in positive samples, and the ftsZ gene sequences were analyzed using BioEdit software. The gene sequence obtained in this study exhibited 100.00 % similarity to reference sequences in the GenBank® database, and a phylogenetic tree was constructed using MEGA11. The results revealed that 15 % of the cats (30 out of 200 blood samples) tested positive for the B. quintana gene, with a 95 % confidence interval of 10.71 % to 20.61 %.
期刊介绍：
Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine .
The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.
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