托马替丁能改善高脂饮食/链脲佐菌素（HFD/STZ）诱导的小鼠 2 型糖尿病。

IF 2.5 4区医学 Q3 ENDOCRINOLOGY & METABOLISM Archives of Physiology and Biochemistry Pub Date : 2024-01-08 DOI:10.1080/13813455.2023.2298404

Li Cai, Baojian Hou, Juping Hu

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引用次数: 0

摘要

目的探讨托马替丁（Td）对小鼠2型糖尿病（T2DM）进展的影响并揭示其机制：方法：通过高脂饮食（HFD）和腹腔注射链脲佐菌素（STZ）诱导 T2DM 小鼠模型。小鼠分组如下1，对照组；2，T2D组；3，T2D+托马替丁（5 mg/kg）组；4，T2D+托马替丁（10 mg/kg）组；5，T2D+托马替丁（20 mg/kg）组。用血糖仪检测空腹血糖，用试剂盒检测空腹胰岛素，以确定 Td 对 T2DM 小鼠的影响。通过 ELISA、qPCR 和免疫印迹检测 Td 对小鼠肝糖稳态和炎症的影响。免疫印迹检测进一步证实了这一机制：结果：Td改善了T2DM小鼠的血糖和胰岛素抵抗。结果：Td 改善了 T2DM 小鼠的血糖和胰岛素抵抗，还改善了 T2DM 小鼠的肝功能和脂质代谢紊乱。Td 还影响了 T2DM 小鼠肝糖稳态相关基因。Td 可减轻 T2DM 小鼠的血清炎症反应。我们进一步发现，Td 激活了 AMPK 通路，从而改善了 T2DM：结论：Td 可改善 HFD/STZ 诱导的小鼠 T2DM，表明它可作为 T2DM 的药物。

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Tomatidine ameliorates high-fat-diet/streptozocin (HFD/STZ)-induced type 2 diabetes mellitus in mice.

Objective: To investigate the effects of tomatidine (Td) on the progression of type 2 diabetes mellitus (T2DM) in mice and uncover the mechanism.

Methods: T2DM mice model was induced by high-fat diet (HFD) and intrabitoneal injection of streptozotocin (STZ). The mice were grouped as follows: 1, control; 2, T2D; 3, T2D + tomatidine (5 mg/kg); 4, T2D + tomatidine (10 mg/kg); 5, T2D + tomatidine (20 mg/kg). Fasting blood glucose was detected by glucose metre and fasting insulin was detected by the kit to determine the effect of Td on T2DM mice. ELISA, qPCR, and Immunoblot assays were performed to detect the effects of Td on the hepatic glucose homeostasis and inflammation of mice. Immunoblot assays further confirmed the mechanism.

Results: Td improved blood glucose and insulin resistance in T2DM mice. In addition, Td improved liver function and lipid metabolism disorder in T2DM mice. Td also affected the liver glucose homeostasis related genes in T2DM mice. Td alleviated serum inflammation in T2DM mice. We further found that Td activated AMPK pathway, therefore ameliorating T2DM.

Conclusion: Td ameliorated HFD/STZ-induced T2DM in mice, suggesting that it could serve as a drug of T2DM.

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来源期刊

Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY

CiteScore

6.90

自引率

3.30%

发文量

期刊介绍： Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.