电针可改善雄性膝骨关节炎兔模型的关节微循环并减轻软骨缺氧症状

IF 3.3 3区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional and Complementary Medicine Pub Date : 2024-07-01 DOI:10.1016/j.jtcme.2024.01.002
Ma Weiwei , Du Mei , Lu Juan , Xing Longfei , Chen Xilin , Hu Tingyao , Zhu Wenting , Guo Changqing
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引用次数: 0

摘要

背景和目的软骨缺氧一直被认为是膝关节骨性关节炎(KOA)的潜在致病因素。研究表明,关节血液灌注受损会导致软骨缺氧。电针(EA)已被证实具有缓解疼痛和改善微循环的作用。本研究旨在探讨 EA 对关节微循环和软骨缺氧的影响及其内在机制。在 KOA 模型建立 3 周后,对膝关节周围的四个点进行 EA 干预。采用勒克森 MG 评分来评估人种学。我们记录了滑膜液的氧张力和体内滑膜微循环。HE染色用于评估软骨形态,免疫组化(IHC)、Western印迹和RT-PCR用于评估主要糖酵解酶葡糖乙转运体1(GLUT1)、丙酮酸激酶M2(PKM2)和乳酸脱氢酶A(LDHA)的表达。结果与结论EA干预后,Lequesne MG评分显著下降,Mankin评分改善(P < 0.01),滑膜微循环(P <0.05)和滑膜液氧张力(P <0.01)明显增加,GLUT1、PKM2、LDHA(P <0.01)和乳酸(P <0.05)的表达明显减少。该研究表明,EA 可通过改善关节微循环和氧张力,改善 KOA 模型兔软骨缺氧状况,调节软骨细胞的糖代谢。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Electroacupuncture improves articular microcirculation and attenuates cartilage hypoxia in a male rabbit model of knee osteoarthritis

Background and aim

Hypoxia of the cartilage has been considered as a potential pathogenic factor in knee osteoarthritis (KOA). Studies have shown that impaired blood perfusion of joint leads to cartilage hypoxia. Electroacupuncture (EA) has proven effects on pain relief and improving microcirculation. This study aimed to explore the effect of EA on articular microcirculation and cartilage anoxic and the underlying mechanisms.

Procedures

Videman's method was used for 6 weeks to establish the KOA model. EA intervention was performed in four points around the knee for 3 weeks after KOA modeling. The Lequesne MG score was used to assess ethology. We recorded the oxygen tension of synovial fluid and the synovial microcirculation in vivo. HE-staining was used to assess cartilage morphology, and immunohistochemistry (IHC), Western blotting, and RT-PCR were used to assess expression of the major glycolytic enzymes glucosetransporter1 (GLUT1), pyruvate kinase M2(PKM2), and lactate dehydrogenase A (LDHA). Enzyme-linked immunosorbent assay (Elisa) was used to detect lactate content.

Results and conclusion

There was a significant decrease in Lequesne MG score and improvement in Mankin score after EA intervention (P < 0.01), a significant increase in synovial microcirculation (P < 0.05) and synovial fluid oxygen tension (P < 0.01), and there was significant decrease in the expression of GLUT1, PKM2 and LDHA (P < 0.01) and lactate (P < 0.05). This study suggested that EA ameliorate cartilage hypoxia and regulate glycolytic metabolism in chondrocytes in KOA model rabbits by improving articular microcirculation and oxygen tension.

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来源期刊
Journal of Traditional and Complementary Medicine
Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine
CiteScore
9.30
自引率
6.70%
发文量
78
审稿时长
66 days
期刊介绍: eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.
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