桑葚提取物和纯化馏分对口腔致病菌的抗菌活性。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Oral Biosciences Pub Date : 2024-06-01 DOI:10.1016/j.job.2023.12.009

Takahisa Shirai , Yutaroh Satoh , Kazuyuki Ishihara

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引用次数: 0

摘要

研究目的本研究旨在从桑叶中分离出对牙周病细菌具有活性的抗菌化合物：方法：采用油水分离法从桑叶油层中提取丙酮可溶部分。提取物经硅胶开柱色谱纯化。每一步都测定了粗提取物或纯化馏分对牙龈卟啉单胞菌的最低抑菌浓度（MIC）：结果：粗提取物对牙龈卟啉单胞菌的最小抑菌浓度为 62.5-125 μg/mL。Cf K 对牙龈脓杆菌、核酸镰刀菌、中间前驱菌和变异链球菌的 MIC 分别为 6.25 μg/mL、25 μg/mL、12.5 μg/mL 和 12.5 μg/mL。相比之下，Cf P 对牙龈球菌、核球菌、中间球菌和变异球菌的 MIC 分别为 25.0 μg/mL、>50 μg/mL、50 μg/mL 和 12.5-25.0 μg/mL：结论：桑叶中含有抗牙周病细菌（如牙龈脓疱病菌、核酸酵母菌和中间脓疱病菌）的抗菌成分。

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Antibacterial activity of mulberry extracts and purified fractions against oral pathogenic bacteria

Objectives

This study aimed to isolate antibacterial compounds active against periodontopathic bacteria from mulberry (Morus alba) leaves.

Methods

The acetone-soluble fraction of mulberry leaves was extracted from the oil layer by oil/water separation. The extract was purified using silica gel open-column chromatography. The minimum inhibitory concentration (MIC) of the crude extract or purified fractions against Porphyromonas gingivalis was measured at each step.

Results

The MIC of the crude extract against P. gingivalis was 62.5–125 μg/mL. The fractions showing activity against P. gingivalis were designated Cf K and Cf P. The MICs of Cf K against P. gingivalis, Fusobacterium nucleatum, Prevotella intermedia, and Streptococcus mutans were 6.25 μg/mL, 25 μg/mL, 12.5 μg/mL, and 12.5 μg/mL, respectively. In contrast, the MICs of Cf P against P. gingivalis, F. nucleatum, P. intermedia, and S. mutans were 25.0 μg/mL, >50 μg/mL, 50 μg/mL, and 12.5–25.0 μg/mL, respectively.