Renpeng Xia , Nan Ouyang , Tingting Wang , Yuan Zhuang , Peng Miao
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引用次数: 0
摘要
谷胱甘肽介导的 MnO2 纳米片还原加速了 CRISPR/Cas12a 系统。通过监测 DNA 探针的反式裂解事件，建立了谷胱甘肽检测的电化学方法，其检测限为 3.5 pM。它为血浆分析提供了一种性能令人满意的工具，表明谷胱甘肽检测法具有广阔的应用前景。
Electrochemical detection of glutathione based on accelerated CRISPR/Cas12a trans-cleavage with MnO2 nanosheets†
The CRISPR/Cas12a system is accelerated by glutathione-mediated reduction of MnO2 nanosheets. By monitoring the trans-cleavage of the DNA probe, an electrochemical method for glutathione assay is fabricated, with the detection limit of 3.5 pM. It provides a promising tool for plasma analysis with satisfactory performance, indicating the broad application prospects of this glutathione assay.
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