{"title":"左旋肉碱通过改变与凋亡和抗凋亡相关的微RNA，有助于提高血小板浓缩物的活力和质量。","authors":"Mozhgan Norouzi , Seyed Alireza Mesbah-Namin , Zohreh Sharifi , Mohammad Reza Deyhim","doi":"10.1016/j.tracli.2024.01.007","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Micro RNAs are known as the main regulator of messenger RNA translation in platelets and have a vital role in process of apoptosis during platelet storage. Our pervious study revealed that the expression of miR-145 and miR-326 changed significantly in platelets under maintenance conditions. This study aimed to evaluate the effect of L-carnitine (LC) as an additive to augment platelet quality by changing the microRNA expression.</p></div><div><h3>Methods</h3><p>We used ten platelet concentrate (PC) bags and divided each into two equal parts, LC- treated, and LC free PC. The expression of miR-145 and miR-326 were determined using real-time PCR. Moreover, we measured platelet count, platelet aggregation, platelet viability, and lactate dehydrogenase activity in all samples.</p></div><div><h3>Results</h3><p>The miR-326 expression significantly increased during platelet storage with mean fold changes of 3.2 for the control and 2.5 for LC- treated PC. The mean fold changes in miR-145 expression was less in the control PC (0.52) compared to the LC- treated PC (0.79). Increased levels of platelet count, platelet aggregation, and platelet viability were found in the LC-treated compared to the untreated PC.</p></div><div><h3>Conclusion</h3><p>LC has a protective effect on platelet apoptosis, reduces the expression of apoptotic microRNA, and prevents the reduction of anti-apoptotic microRNA.</p></div>","PeriodicalId":23262,"journal":{"name":"Transfusion Clinique et Biologique","volume":"31 2","pages":"Pages 87-94"},"PeriodicalIF":1.4000,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"L-carnitine contributes to enhancement of viability and quality of platelet concentrates through changing the apoptotic and anti-apoptotic associated microRNAs\",\"authors\":\"Mozhgan Norouzi , Seyed Alireza Mesbah-Namin , Zohreh Sharifi , Mohammad Reza Deyhim\",\"doi\":\"10.1016/j.tracli.2024.01.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Micro RNAs are known as the main regulator of messenger RNA translation in platelets and have a vital role in process of apoptosis during platelet storage. Our pervious study revealed that the expression of miR-145 and miR-326 changed significantly in platelets under maintenance conditions. This study aimed to evaluate the effect of L-carnitine (LC) as an additive to augment platelet quality by changing the microRNA expression.</p></div><div><h3>Methods</h3><p>We used ten platelet concentrate (PC) bags and divided each into two equal parts, LC- treated, and LC free PC. The expression of miR-145 and miR-326 were determined using real-time PCR. Moreover, we measured platelet count, platelet aggregation, platelet viability, and lactate dehydrogenase activity in all samples.</p></div><div><h3>Results</h3><p>The miR-326 expression significantly increased during platelet storage with mean fold changes of 3.2 for the control and 2.5 for LC- treated PC. The mean fold changes in miR-145 expression was less in the control PC (0.52) compared to the LC- treated PC (0.79). Increased levels of platelet count, platelet aggregation, and platelet viability were found in the LC-treated compared to the untreated PC.</p></div><div><h3>Conclusion</h3><p>LC has a protective effect on platelet apoptosis, reduces the expression of apoptotic microRNA, and prevents the reduction of anti-apoptotic microRNA.</p></div>\",\"PeriodicalId\":23262,\"journal\":{\"name\":\"Transfusion Clinique et Biologique\",\"volume\":\"31 2\",\"pages\":\"Pages 87-94\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-01-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Transfusion Clinique et Biologique\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1246782024000077\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"HEMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transfusion Clinique et Biologique","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1246782024000077","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"HEMATOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景：众所周知，微小 RNA 是血小板中信使 RNA 翻译的主要调节因子，在血小板储存过程中的凋亡过程中起着至关重要的作用。我们之前的研究发现，在维持条件下，血小板中 miR-145 和 miR-326 的表达发生了显著变化。本研究旨在评估左旋肉碱（LC）作为添加剂通过改变微RNA的表达来提高血小板质量的效果：我们使用了 10 袋血小板浓缩物（PC），并将每袋分为两等份，分别是经 LC 处理的 PC 和不含 LC 的 PC。使用实时 PCR 测定 miR-145 和 miR-326 的表达。此外，我们还测定了所有样本的血小板计数、血小板聚集、血小板活力和乳酸脱氢酶活性：结果：在血小板储存期间，miR-326 的表达量明显增加，对照组的平均变化倍数为 3.2，经 LC 处理的 PC 的平均变化倍数为 2.5。与 LC 处理的 PC（0.79）相比，对照 PC 中 miR-145 表达的平均折叠变化较小（0.52）。与未处理的 PC 相比，经 LC 处理的 PC 的血小板计数、血小板聚集和血小板活力水平均有所提高：结论：LC 对血小板凋亡有保护作用，可减少凋亡微RNA的表达，并防止抗凋亡微RNA的减少。
L-carnitine contributes to enhancement of viability and quality of platelet concentrates through changing the apoptotic and anti-apoptotic associated microRNAs
Background
Micro RNAs are known as the main regulator of messenger RNA translation in platelets and have a vital role in process of apoptosis during platelet storage. Our pervious study revealed that the expression of miR-145 and miR-326 changed significantly in platelets under maintenance conditions. This study aimed to evaluate the effect of L-carnitine (LC) as an additive to augment platelet quality by changing the microRNA expression.
Methods
We used ten platelet concentrate (PC) bags and divided each into two equal parts, LC- treated, and LC free PC. The expression of miR-145 and miR-326 were determined using real-time PCR. Moreover, we measured platelet count, platelet aggregation, platelet viability, and lactate dehydrogenase activity in all samples.
Results
The miR-326 expression significantly increased during platelet storage with mean fold changes of 3.2 for the control and 2.5 for LC- treated PC. The mean fold changes in miR-145 expression was less in the control PC (0.52) compared to the LC- treated PC (0.79). Increased levels of platelet count, platelet aggregation, and platelet viability were found in the LC-treated compared to the untreated PC.
Conclusion
LC has a protective effect on platelet apoptosis, reduces the expression of apoptotic microRNA, and prevents the reduction of anti-apoptotic microRNA.
期刊介绍：
Transfusion Clinique et Biologique, the official journal of the French Society of Blood Transfusion (SFTS):
- an aid to training, at a European level
- the only French journal indexed in the hematology and immunology sections of Current Contents
Transfusion Clinique et Biologique spans fundamental research and everyday practice, with articles coming from both sides. Articles, reviews, case reports, letters to the editor and editorials are published in 4 editions a year, in French or in English, covering all scientific and medical aspects of transfusion: immunology, hematology, infectious diseases, genetics, molecular biology, etc. And finally, a convivial cross-disciplinary section on training and information offers practical updates.
Readership:
"Transfusers" are many and various: anesthetists, biologists, hematologists, and blood-bank, ICU and mobile emergency specialists...
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