鸡胚结肠杯状细胞粘蛋白的组织化学分析。

G Gheri, S Gheri Bryk
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引用次数: 0

摘要

研究了鸡胚结肠上皮粘蛋白的组织化学特性。在培养的第14天,可以证实糖原的存在。第15天,少量上皮细胞出现中性和唾液化的粘蛋白。第16天,在典型杯状细胞中检测到硫酸酸化的分泌物质以及中性和唾液化的粘蛋白。从第17天到第20天,在一些细胞中出现了两种类型的酸性粘蛋白,并放置在核上细胞质的不同区域。在第21天,大多数杯状细胞中均存在中性、唾液化和硫酸盐化的粘蛋白,主要存在于隐窝上皮内。
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Histochemical analysis of goblet cell mucins in the chick embryo colon.

The histochemical characteristics of colonic epithelial mucins were investigated in the chick embryo. At the 14th day of incubation it was possible to demonstrate the presence of glycogen. At the 15th day a few epithelial cells showed the presence of neutral and sialylated mucins. On the 16th day, also sulfated secretory material was detectable together with neutral and sialylated mucins in cells with the typical shape of goblet cells. From the 17th day to the 20th day of incubation the two types of acid mucins appeared in some cells to be placed in distinct zones of the supranuclear cytoplasm. At the 21st day, neutral, sialylated and sulfated mucins were all present in the majority of goblet cells, which were found mainly in the epithelium lining the crypts.

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