Santiago Nicolás Freytes, María Laura Gobbini, Pablo D Cerdán
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引用次数: 0
摘要
自从在酵母中发现媒介复合体以来,30 年已经过去了。我们目睹了各种突破和进展,这些突破和进展产生了酵母和哺乳动物启动前复合体中调解子的高分辨率结构模型,展示了它是如何组装的,以及它是如何定位 RNA 聚合酶 II 及其 C 端结构域 (CTD) 以促进 CTD 磷酸化从而启动转录的。这些信息也可用于指导未来有关 Mediator 转录控制机制的植物研究。在此,我们回顾了我们所了解的植物介导子的亚基组成和结构、各个亚基的作用和开创介导子研究的遗传分析,以及转录因子如何将介导子招募到与启动子相邻的调控区域。研究结果表明,Mediator 可调节转录活性,并招募激素信号模块和组蛋白修饰活动,以建立一种关闭或开启的转录状态,从而招募一般转录因子进行启动前复合体组装。植物生物学年刊》(Annual Review of Plant Biology)第 75 卷的最终在线出版日期预计为 2024 年 5 月。修订后的预计日期请参见 http://www.annualreviews.org/page/journal/pubdates。
The Plant Mediator Complex in the Initiation of Transcription by RNA Polymerase II.
Thirty years have passed since the discovery of the Mediator complex in yeast. We are witnessing breakthroughs and advances that have led to high-resolution structural models of yeast and mammalian Mediators in the preinitiation complex, showing how it is assembled and how it positions the RNA polymerase II and its C-terminal domain (CTD) to facilitate the CTD phosphorylation that initiates transcription. This information may be also used to guide future plant research on the mechanisms of Mediator transcriptional control. Here, we review what we know about the subunit composition and structure of plant Mediators, the roles of the individual subunits and the genetic analyses that pioneered Mediator research, and how transcription factors recruit Mediators to regulatory regions adjoining promoters. What emerges from the research is a Mediator that regulates transcription activity and recruits hormonal signaling modules and histone-modifying activities to set up an off or on transcriptional state that recruits general transcription factors for preinitiation complex assembly.
期刊介绍:
The Annual Review of Plant Biology is a peer-reviewed scientific journal published by Annual Reviews. It has been in publication since 1950 and covers significant developments in the field of plant biology, including biochemistry and biosynthesis, genetics, genomics and molecular biology, cell differentiation, tissue, organ and whole plant events, acclimation and adaptation, and methods and model organisms. The current volume of this journal has been converted from gated to open access through Annual Reviews' Subscribe to Open program, with all articles published under a CC BY license.