在营养中使用 "保护 "型甜菜碱时羊的消化过程和肠道内容物的微生物变化

М. Pavlova, N. Bogolyubova, V. Romanov, О. Artemyeva
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摘要

在维持反刍动物胃肠道代谢过程的过程中,一个重要环节是确保完整的饲粮。对使用 "保护 "形式的甜菜碱补充剂以避免瘤胃微生物菌群的间接影响的研究表明,甜菜碱对绵羊的消化过程、瘤胃和肠道的微生物组成具有积极的作用。在以科学院院士 L.K. Ernst 命名的联邦畜牧科学中心生理试验场的条件下,对年龄为 8-9 个月的罗曼诺夫品种和卡塔丁品种杂交绵羊进行了生理试验,这些绵羊根据巴索夫(n=3)的说法患有慢性疤痕瘘。在对照期,动物摄入主食;在实验 1 期,动物摄入主食 + 未受保护的甜菜碱,剂量为每公斤活重 0.05 克;在实验 2 期,动物摄入 "受保护的 "甜菜碱补充剂,剂量为每公斤活重 0.05 克。每个实验期为 30 天。在核算期间,按照公认的方法平均每天采集粪便样本。为了研究卡他性消化,在每个阶段结束时,通过巴索夫的疤痕瘘管从动物体内采集内容物样本。使用 "保护 "型和原生型甜菜碱的结果是,疤痕内容物中微生物的数量高于对照组。在动物日粮中添加 "保护 "型甜菜碱后,饲料养分的总体消化率趋于提高。在第 2 个实验期,与对照组相比,绵羊大肠中双歧杆菌的含量增加了 1.63 CFU/g,致病性酵母真菌的含量下降了数倍。
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Digestive processes and microbiocenosis of intestinal contents in sheep when using a «protected» form of betaine in nutrition
In the process of maintaining the metabolic processes of the gastrointestinal tract of ruminants, an important link is to ensure a complete feeding diet. The study of the use of betaine supplements in a form "protected" from the indirect effects of the microflora of the rumen showed positive dynamics in relation to the digestive processes, the microbial composition of the rumen and the intestines of sheep. In the conditions of the physiological yard of the Federal Science Center for Animal Husbandry named after Academy Member L.K. Ernst conducted a physiological experiment on ramshanks of crossbreeds of the Romanov breed and katadins, aged 8-9 months, with chronic scar fistulas according to Basov (n=3). In the control period, the animals received the main diet, in the experimental 1 – the main diet + unprotected betaine at a dosage of 0.05 g / kg of live weight, in the 2nd experimental period a "protected" betaine supplement at a dosage of 0.05 g / kg of live weight. The duration of each period was 30 days. During the accounting period, average daily stool samples were taken according to the generally accepted method. To study cicatricial digestion, at the end of each period, samples of the contents were taken from animals through the fistula of the scar by Basov. As a result of the use of both the "protected" and native forms of betaine, the mass of microorganisms of the scar content became higher than in the control period. When the "protected" form of betaine was added to the diet of animals, the overall digestibility of feed nutrients tended to improve. In the 2nd experimental period, compared with the control, the level of bifidobacteria in the large intestine of sheep increased by 1.63 CFU/g, the level of pathogenic yeast fungi decreased several times.
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