韩国Albizia kalkora(豆科)的完整叶绿体基因组及其分类学定位

Pub Date : 2024-01-18 DOI:10.1016/j.japb.2023.12.009
Jong-Soo Park , Youngsung Kim , Iseon Kim , Ho-Rim Hwang , Changkyun Kim
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引用次数: 0

摘要

Albizia kalkora (Roxb.) Prain(豆科)是一种温带和亚热带落叶乔木,分布于东亚、东南亚和印度。然而,韩国 A. kalkora 在分类学上的地位仍不明确。在此,我们提供了韩国A. kalkora的完整叶绿体(cp)基因组,以明确其在分类学上的地位以及在该属中的系统发育关系。cp 基因组是基于 Illumina HiSeq 读数组装的,长度为 176,402 bp。A. kalkora cp 基因组的 GC 含量为 35.4%。A. kalkora的cp基因组显示出典型的四方结构,由一对倒位重复序列(39 716 bp)和大单拷贝区(91 862 bp)及小单拷贝区(5 108 bp)分隔而成。利用基于 73 个蛋白编码基因的最大似然法和贝叶斯推断法构建的系统发生树显示,A. kalkora 位于 Albizia 支系内,而 Albizia 支系是 Archidendron 支系的姊妹支系。出乎意料的是,我们发现韩国 A. kalkora 是 Albizia julibrissin 的姊妹,而不是来自中国的 A. kalkora 个体。我们所获得的A. kalkora的完整cp基因组数据有助于通过开发物种特异性分子标记来鉴定物种,从而为物种保护提供指导。
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Complete chloroplast genome of Albizia kalkora (Fabaceae) in Korea and its taxonomic position

Albizia kalkora (Roxb.) Prain (Fabaceae) is a temperate and subtropical deciduous tree distributed in East and Southeast Asia and India. However, the taxonomic position of the Korean A. kalkora remains unclear. Here, we provide the complete chloroplast (cp) genome of the Korean A. kalkora to clarify its taxonomic position and phylogenetic relationship within the genus. The cp genome was assembled based on Illumina HiSeq reads and was 176,402 bp in length. The GC content of A. kalkora cp genome was 35.4%. The cp genome of A. kalkora revealed a typical quadripartite structure consisting of a pair of inverted repeats (39,716 bp) separated by the large single copy (91,862 bp) and small single copy (5,108 bp) regions. The phylogenetic trees constructed using the maximum likelihood and Bayesian inference methods based on 73 protein-coding genes revealed that A. kalkora is positioned within the Albizia clade, which is a sister to the Archidendron clade. Unexpectedly, the accession of the Korean A. kalkora was found to be a sister to Albizia julibrissin instead of an individual of the A. kalkora from China. Our complete cp genome data of A. kalkora are useful for identifying species by developing species-specific molecular markers, thereby providing a guideline for conservation.

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