富含半胱氨酸的血管生成诱导因子 61 与 MicroRNA -155 的表达比值是子痫前期的诊断指标和严重程度的预测指标。

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-07-01 DOI:10.61186/rbmb.12.2.332

Sarah Mamdouh Shoeib, Doaa Elwy Abdeldaim, Shaimaa Samir Mashal, Rowida Raafat Ibrahim, Lamees Mohamed Dawood, Doaa Shatat, Yasmine Ibrahim El-Masry, Ahmed Almeldin, Radwa Mahamoud El Sharaby

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引用次数: 0

摘要

背景：子痫前期（PE）是一种多系统妊娠疾病，会显著增加母婴发病率和死亡率。孕妇血清中的微RNA-155（miR-155）过表达与子痫前期有关。研究人员发现，miR-155 在妊娠期间通过下调和减少富半胱氨酸血管生成诱导剂 61（CYR61）发挥作用，而 CYR61 会导致局部缺血和氧化应激：方法：使用实时聚合酶链反应（RT-PCR）对所有血清样本中 miR-155 的表达水平进行定量，并使用酶联免疫吸附试验测定血清 CYR61。它们与 Cyr-61/miR-155 的比值一起被评估为 PE 发病机制和严重程度预测的生物标志物：结果：与对照组相比，PE 患者的 MiR-155 表达、血清 CYR61 水平和 Cyr-61/miR-155 比率均显著升高。轻度和重度 PE 患者的血清 CYR61 水平和 Cyr-61/miR-155 比值有明显差异：结论：MiR-155表达、血清CYR61水平和Cyr-61/miR-155可作为PE发病机制和严重程度预测的生物标志物。

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The Ratio of Cysteine-Rich Angiogenic Inducer 61 to MicroRNA -155 Expression as a Preeclampsia Diagnostic Marker and Predictor of Its Severity.

Background: Preeclampsia (PE) is a multisystem pregnancy disorder that increases maternal-perinatal morbidity and mortality significantly. MicroRNA-155 (miR-155) overexpression in the sera of pregnant women has been linked to preeclampsia. Researchers discovered that miR-155 acts during pregnancy by down-regulating and reducing the cysteine-rich angiogenic inducer 61 (CYR61), which causes local ischemia as well as oxidative stress.

Methods: The level of miR-155 expression in all serum samples was quantified using real-time polymerase chain reaction (RT-PCR), and serum CYR61 was measured using enzyme-linked immunosorbent assays. Together with the Cyr-61/miR-155 ratio, they were evaluated as biomarkers for PE pathogenesis and severity prediction.

Results: MiR-155 expression, serum CYR61 levels, and Cyr-61/miR-155 ratios were all significantly higher in PE patients compared to the control group. Serum CYR61 levels and the Cyr-61/miR-155 ratio differed significantly between mild and severe PE patients.

Conclusions: MiR-155 expression, serum CYR61 levels, and Cyr-61/miR-155 may serve as biomarkers for PE pathogenesis and severity prediction.

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来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.