体外比较研究松脂醇和 Lariciresinol 对健康细胞和乳腺癌衍生人类细胞的抗肿瘤作用。

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL Iranian Journal of Medical Sciences Pub Date : 2024-01-01 DOI:10.30476/ijms.2023.94805.2611
Mona Soltani, Reza Fotovat, Mohsen Sharifi, Najmeh Ahmadian Chashmi, Mehrdad Behmanesh
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引用次数: 0

摘要

背景:由于中草药对健康细胞的细胞毒性较低,因此是首选的抗癌药物。植物木脂素在治疗各种疾病,尤其是癌症方面发挥着重要作用。本研究旨在评估豆荚素、松脂醇和落叶松脂醇对成纤维细胞、HEK-293 和 SkBr3 细胞系的细胞毒性和诱导细胞凋亡的影响:2017 年至 2019 年,在伊朗德黑兰 Tarbiat Modares 大学生物科学系进行了一项体外研究。细胞株分别用不同浓度的木酚素处理 24 小时和 48 小时。分别使用 MTT 和流式细胞仪检测细胞活力和凋亡。细胞周期和细胞凋亡调节基因的表达水平采用实时定量聚合酶链反应法进行测定。数据采用双向方差分析,然后进行 Tukey's HSD 检验。结果与松脂醇和落叶松脂醇相比,鬼臼毒素能明显增加成纤维细胞的凋亡:松脂醇和落叶松脂醇有可能被用作治疗乳腺癌的新药。
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In Vitro Comparative Study on Antineoplastic Effects of Pinoresinol and Lariciresinol on Healthy Cells and Breast Cancer-Derived Human Cells.

Background: Herbal medicines are the preferred anticancer agents due to their lower cytotoxic effects on healthy cells. Plant lignans play an important role in treating various diseases, especially cancer. The present study aimed to evaluate the effect of podophyllotoxin, pinoresinol, and lariciresinol on cellular toxicity and inducing apoptosis in fibroblasts, HEK-293, and SkBr3 cell lines.

Methods: An in vitro study was conducted from 2017 to 2019 at the Faculty of Biological Sciences, Tarbiat Modares University (Tehran, Iran). The cell lines were treated for 24 and 48 hours with different concentrations of lignans. Cell viability and apoptosis were examined using MTT and flow cytometry, respectively. Expression levels of cell cycle and apoptosis regulator genes were determined using quantitative real-time polymerase chain reaction. Data were analyzed using a two-way analysis of variance followed by Tukey's HSD test. P<0.05 was considered statistically significant.

Results: Podophyllotoxin significantly increased apoptosis in fibroblast cells compared to pinoresinol and lariciresinol (P<0.001). The percentage of cell viability of fibroblast cells treated for 48 hours with pinoresinol, lariciresinol, and podophyllotoxin was reduced by 49%, 47%, and 36%, respectively. Treatment with pinoresinol and lariciresinol significantly overexpressed pro-apoptotic genes and underexpressed anti-apoptotic genes in SkBr3 cells (P<0.001). SkBr3 cells treated with lariciresinol significantly reduced gene expression (P<0.001).

Conclusion: Pinoresinol and lariciresinol can potentially be used as new therapeutic agents for the treatment of breast cancer.

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来源期刊
Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
0.00%
发文量
84
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of com­muni­cation for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science re­search experiences on prevalent diseases in the region and analysis of various regional problems.
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