结直肠癌患者体内 IRE1-XBP1s、p62 和 Caspase-3 的表达及临床意义

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL Iranian Journal of Medical Sciences Pub Date : 2024-01-01 DOI:10.30476/IJMS.2023.96922.2856
Mohammadkian Zarafshani, Habibollah Mahmoodzadeh, Vahid Soleimani, Mohammad Amin Moosavi, Marveh Rahmati
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引用次数: 0

摘要

背景:包括内质网应激反应(ERS)、自噬和细胞凋亡在内的三大细胞信号通路在细胞存活和死亡过程中发挥着关键作用。研究发现,在肿瘤发生和癌症进展过程中,这些信号通路会相互影响。本研究旨在探讨作为ERS、自噬和细胞凋亡重要生物标志物的X-box结合蛋白1(XBP1s)剪接形式、p62和caspase-3在结直肠癌(CRC)患者中的表达情况,以及它们的表达与临床病理数据之间的相关性:这项回顾性研究的对象是福尔马林固定石蜡包埋(FFPE)区块,这些区块是从伊玛目霍梅尼医院(伊朗德黑兰)肿瘤库中收集的患者及其肿瘤边缘。组织芯片(TMA)用于测量XBP1s、p62和caspase-3生物标志物。数据采用SPSS软件20版进行分析,P≤0.05为差异有统计学意义:对91例患者进行评估后发现,XBP1s的表达与TNM分期(P=0.003)、原发肿瘤(pT)(P=0.054)和分化程度(P=0.006)有显著关系;caspase-3与pT(P=0.004)和淋巴管侵犯(P=0.02)有显著关系。然而,p62 与临床病理数据之间没有发现明显的相关性。此外,XBP1s与p62之间的正相关关系得到了证实(相关系数:22.2%,P=0.05):我们的研究结果表明,XBP1s可被视为个体化医疗的治疗靶点。
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Expression and Clinical Significance of IRE1-XBP1s, p62, and Caspase-3 in Colorectal Cancer Patients.

Background: Three main cell signaling pathways including the endoplasmic reticulum stress (ERS) response, autophagy, and apoptosis play critical roles in both cell survival and death. They were found to crosstalk with one another during tumorigenesis and cancer progression. This study aimed to investigate the expression of the spliced form of X-box binding protein 1 (XBP1s), p62, and caspase-3, as the essential biomarkers of ERS, autophagy, and apoptosis in patients with colorectal cancer (CRC), as well as the correlation between their expression and clinicopathological data.

Methods: This retrospective study was conducted on formalin-fixed paraffin-embedded (FFPE) blocks, which were collected from patients and their tumor margins, from the tumor bank of Imam Khomeini Hospital (Tehran, Iran) from 2017 to 2019. Tissue microarray (TMA) was used to measure the XBP1s, p62, and caspase-3 biomarkers. Data were analyzed using SPSS software version 20, and P≤0.05 was considered statistically significant.

Results: Evaluating the total of 91 patients, a significant relationship was found between XBP1s expression and TNM stage (P=0.003), primary tumor (pT) (P=0.054), and the degree of differentiation (P=0.006); and between caspase-3 with pT (P=0.004), and lymphovascular invasion (P=0.02). However, no significant correlation was found between p62 and clinicopathological data. Furthermore, a positive relationship between XBP1s and p62 was confirmed (correlation coefficient: 22.2% and P=0.05).

Conclusion: Our findings indicated that XBP1s could be considered as a target for therapy in personalized medicine.

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来源期刊
Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
0.00%
发文量
84
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of com­muni­cation for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science re­search experiences on prevalent diseases in the region and analysis of various regional problems.
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