采血针中的死腔对血友病患儿 FVIII 水平和药代动力学特征的影响。

IF 2 4区 医学 Q3 HEMATOLOGY Hematology Pub Date : 2024-12-01 Epub Date: 2024-02-12 DOI:10.1080/16078454.2024.2314871
Yingzi Zhen, Di Ai, Kun Huang, Gang Li, Zhenping Chen, Runhui Wu
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引用次数: 0

摘要

目的研究一次性采血针的死腔对血友病患儿活化部分凝血活酶时间(APTT)、FVIII 水平和药代动力学(PK)曲线的影响:儿童(1/2)、清除率(CL)、谷值和剂量为 50 ± 10IU/dL 后达到 1、2 和 5IU/dL 的时间。将输注后 9 小时和 24 小时的 FVIII 活性输入 WAPPS,从而得出四种真实或偏差 FVIII 水平的组合:结果:与标准采集血样相比,9H-S&24H-S 组和 9H-NS&24H-S 组的 APTT 结果延长(P 值 P 值 P P P P 1/2),CL 值降低,达到 1、2 或 5IU/dL 的时间延长:结论:在使用真空管检测凝血指标和 PK 图谱时,应提前消除采血针的死角。
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The influence of dead space in blood sampling needle on FVIII level and pharmacokinetic profiles in children with hemophilia.

Objective: To investigate the influence of the dead space in disposable blood sampling needle on activated partial thromboplastin time (APTT), FVIII level and pharmacokinetic (PK) profiles in children with hemophilia.

Methods: Children (<18 years) with severe hemophilia A were enrolled. After three days' washout-period, blood samples were collected at pre-dose, 1 h, 3 h, 9 h, 24 h and 48 h post-infusion. At each timepoint, two 2 mL vacuum tubes with 3.2% trisodium citrate were used. The first tube was signed as 'non-standard' (NS) and the second tube was signed as 'standard' (S). FVIII activities were evaluated by one-stage assay. WAPPS-Hemo was used to generate PK profiles like half-life time (t1/2), clearance (CL), trough level and time to 1, 2 and 5IU/dL after a dose of 50 ± 10IU/dL. The FVIII activities at 9 h and 24 h post-infusion were put into WAPPS and thus brought four combinations by true or biased FVIII level that used.

Result: Compared with standard-collected blood samples, prolonged APTT results (P-values < 0.01) and decreased FVIII activity (P-values < 0.05) were revealed in those non-standard blood samples. The corresponding bias was in positive relation to both APTT-S (r = 0.44, P < 0.0001) and FVIII-S level(r = 0.68, P < 0.001). The FVIII bias percentage got larger as FVIII-S level reduced (r = -0.24, P < 0.01). During the four combinations of FVIII activity at 9 h and 24 h, statistically longer t1/2, lower CL and longer time to 1, 2 or 5IU/dL were observed in 9H-S&24H-S group and 9H-NS&24H-S group.

Conclusion: While using vacuum tubes for clotting indicators and PK profiles, the dead space of blood sampling needle should be eliminated in advance.

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来源期刊
Hematology
Hematology 医学-血液学
CiteScore
2.60
自引率
5.30%
发文量
140
审稿时长
3 months
期刊介绍: Hematology is an international journal publishing original and review articles in the field of general hematology, including oncology, pathology, biology, clinical research and epidemiology. Of the fixed sections, annotations are accepted on any general or scientific field: technical annotations covering current laboratory practice in general hematology, blood transfusion and clinical trials, and current clinical practice reviews the consensus driven areas of care and management.
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