检测结核分枝杆菌的 S 基因型并澄清欧美血统中有争议的 Spoligotypes 的位置

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-02-07 DOI:10.1134/s0026261723603457

I. V. Mokrousov, S. N. Zhdanova, V. Valcheva, G. I. Alekseeva, M. K. Vinokurova, N. E. Evdokimova, O. B. Ogarkov

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引用次数: 0

摘要

摘要--S 基因型（首次描述于西西里岛，因此得名）是欧美结核分枝杆菌系的一部分。在此，我们报告了一种基于 Rv0557 基因 PCR-RFLP 的检测方法。该方法在已知全基因组序列的菌株上进行了优化，并进一步应用于筛选来自俄罗斯、保加利亚、中国、日本和越南的 DNA 收集物。PCR-RFLP 分析证实了具有 Spoligotypes SIT34（S 的祖先）和 SIT1253（雅库特菌株）的菌株的 S 基因型。来自保加利亚的菌株具有 SIT4 和 SIT125 的节略 spoligoprofiles，也被归入 S 基因型。所开发的方法可快速检测结核杆菌的 S 基因型，并可靠地解决有争议的 spoligotypes 系统发育模糊问题。

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Detection of the Mycobacterium tuberculosis S-Genotype and Clarification of the Position of Controversial Spoligotypes of the Euro-American Lineage

Abstract—

The S genotype (first described in Sicily, hence the name) is a part of the Euro-American lineage of Mycobacterium tuberculosis. Here, we report a method for its detection based on PCR-RFLP of the Rv0557 gene. The method was optimized on strains with known whole genome sequences and further applied to screening the DNA collections from Russia, Bulgaria, China, Japan, and Vietnam. PCR-RFLP analysis confirmed the S genotype in strains with the spoligotypes SIT34 (ancestral to S) and SIT1253 (Yakutia strains). Strains from Bulgaria with abridged spoligoprofiles SIT4 and SIT125 were also assigned to the S genotype. The developed method provides for rapid detection of the M. tuberculosis S genotype and reliably resolves phylogenetic ambiguity of the disputed spoligotypes.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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