角膜内皮细胞与基质细胞在角膜内皮损伤过程中的相互作用

Kyung Wook Kim, Young Joo Shin
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摘要

目的:研究角膜内皮细胞(CECs)与角膜基质细胞(CSCs)的相互作用对化学性角膜内皮损伤愈合的影响:将 25 只兔子分为两组:暴露于 NaOH 的兔子和对照组。分别于角膜内皮损伤后 1 天、1 周、2 周和 4 周对兔子进行临床评估并处死。在 Transwell 系统中培养角膜内皮细胞和角膜基质细胞,分析共培养和不共培养角膜内皮细胞的伤口愈合率和培养液中的细胞因子:结果:所有暴露于 NaOH 的兔子的角膜水肿和翳评分均高于对照组。在暴露于 NaOH 的第一天,观察到兔子角膜内皮凋亡,但对 CSCs 影响不大。损伤两周后,CECs 从周边迁移。CSCs与Descemet膜平行排列,显示出CECs的存在。共培养系统中转化因子-β1和白细胞介素-6的分泌量低于单独培养的CECs或CSCs:这些结果表明,巩膜内注射 NaOH 是一种角膜内皮损伤模型,对基质的影响极小。在伤口愈合过程中,CECs 和 CSCs 之间的交流可能会导致细胞因子的产生,而细胞因子的产生是增强角膜内皮损伤愈合过程所必需的。
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Interactions of Corneal Endothelial Cells with Stromal Cells during Corneal Endothelial Injury
Purpose: To investigate the interaction of corneal endothelial cells (CECs) with corneal stromal cells (CSCs) on the healing of chemically induced corneal endothelial injury.Methods: Twenty-five rabbits were divided into two groups: rabbits exposed to NaOH and controls. Rabbits were clinically evaluated and sacrificed at 1 day, 1 week, 2 weeks, and 4 weeks after endothelial injury. Corneal endothelial cells and corneal stromal cells were cultured and the wound healing rate of the corneal endothelium and cytokines in the culture medium were analyzed with and without co-culture in the Transwell system.Results: Corneal edema and opacity scores were higher in all rabbits exposed to NaOH than in controls. Corneal endothelial apoptosis with little effect on CSCs was observed in rabbits exposed to NaOH at day 1. Two weeks after injury, CECs migrated from the periphery. CSCs were aligned in parallel to Descemet's membrane, which exhibited the presence of CECs. Transforming factor-β1 and interleukin-6 secretion was lower in the co-culture system than in individual cultures of CECs or CSCs.Conclusions: These results revealed intracameral NaOH injection is a model of corneal endothelial injury with minimal impact on the stroma. Communication between CECs and CSCs during wound healing may lead to the modulation of cytokine production, which is necessary for an enhanced healing process of corneal endothelial injury.
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