{"title":"利用原生土壤接种菌进行链延伸:特殊的正己酸酯生物合成性能和微生物机制","authors":"","doi":"10.1016/j.eng.2023.10.017","DOIUrl":null,"url":null,"abstract":"<div><p>This study demonstrates the feasibility and effectiveness of utilizing native soils as a resource for inocula to produce <em>n</em>-caproate through the chain elongation (CE) platform, offering new insights into anaerobic soil processes. The results reveal that all five of the tested soil types exhibit CE activity when supplied with high concentrations of ethanol and acetate, highlighting the suitability of soil as an ideal source for <em>n</em>-caproate production. Compared with anaerobic sludge and pit mud, the native soil CE system exhibited higher selectivity (60.53%), specificity (82.32%), carbon distribution (60.00%), electron transfer efficiency (165.00%), and conductivity (0.59 ms∙cm<sup>−1</sup>). Kinetic analysis further confirmed the superiority of soil in terms of a shorter lag time and higher yield. A microbial community analysis indicated a positive correlation between the relative abundances of <em>Pseudomonas</em>, <em>Azotobacter</em>, and <em>Clostridium</em> and <em>n</em>-caproate production. Moreover, metagenomics analysis revealed a higher abundance of functional genes in key microbial species, providing direct insights into the pathways involved in <em>n</em>-caproate formation, including <em>in situ</em> CO<sub>2</sub> utilization, ethanol oxidation, fatty acid biosynthesis (FAB), and reverse beta-oxidation (RBO). The numerous functions in FAB and RBO are primarily associated with <em>Pseudomonas</em>, <em>Clostridium</em>, <em>Rhodococcus</em>, <em>Stenotrophomonas</em>, and <em>Geobacter</em>, suggesting that these genera may play roles that are involved or associated with the CE process. Overall, this innovative inoculation strategy offers an efficient microbial source for <em>n</em>-caproate production, underscoring the importance of considering CE activity in anaerobic soil microbial ecology and holding potential for significant economic and environmental benefits through soil consortia exploration.</p></div>","PeriodicalId":11783,"journal":{"name":"Engineering","volume":"39 ","pages":"Pages 262-272"},"PeriodicalIF":10.1000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095809924000602/pdfft?md5=09e7b0784d0ba3c2de89df06979dab74&pid=1-s2.0-S2095809924000602-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Chain Elongation Using Native Soil Inocula: Exceptional n-Caproate Biosynthesis Performance and Microbial Mechanisms\",\"authors\":\"\",\"doi\":\"10.1016/j.eng.2023.10.017\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study demonstrates the feasibility and effectiveness of utilizing native soils as a resource for inocula to produce <em>n</em>-caproate through the chain elongation (CE) platform, offering new insights into anaerobic soil processes. The results reveal that all five of the tested soil types exhibit CE activity when supplied with high concentrations of ethanol and acetate, highlighting the suitability of soil as an ideal source for <em>n</em>-caproate production. Compared with anaerobic sludge and pit mud, the native soil CE system exhibited higher selectivity (60.53%), specificity (82.32%), carbon distribution (60.00%), electron transfer efficiency (165.00%), and conductivity (0.59 ms∙cm<sup>−1</sup>). Kinetic analysis further confirmed the superiority of soil in terms of a shorter lag time and higher yield. A microbial community analysis indicated a positive correlation between the relative abundances of <em>Pseudomonas</em>, <em>Azotobacter</em>, and <em>Clostridium</em> and <em>n</em>-caproate production. Moreover, metagenomics analysis revealed a higher abundance of functional genes in key microbial species, providing direct insights into the pathways involved in <em>n</em>-caproate formation, including <em>in situ</em> CO<sub>2</sub> utilization, ethanol oxidation, fatty acid biosynthesis (FAB), and reverse beta-oxidation (RBO). The numerous functions in FAB and RBO are primarily associated with <em>Pseudomonas</em>, <em>Clostridium</em>, <em>Rhodococcus</em>, <em>Stenotrophomonas</em>, and <em>Geobacter</em>, suggesting that these genera may play roles that are involved or associated with the CE process. Overall, this innovative inoculation strategy offers an efficient microbial source for <em>n</em>-caproate production, underscoring the importance of considering CE activity in anaerobic soil microbial ecology and holding potential for significant economic and environmental benefits through soil consortia exploration.</p></div>\",\"PeriodicalId\":11783,\"journal\":{\"name\":\"Engineering\",\"volume\":\"39 \",\"pages\":\"Pages 262-272\"},\"PeriodicalIF\":10.1000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2095809924000602/pdfft?md5=09e7b0784d0ba3c2de89df06979dab74&pid=1-s2.0-S2095809924000602-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2095809924000602\",\"RegionNum\":1,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2095809924000602","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
这项研究证明了利用原生土壤作为接种物资源,通过链延伸(CE)平台生产正己酸酯的可行性和有效性,为了解厌氧土壤过程提供了新的视角。结果表明,当提供高浓度乙醇和乙酸时,所有五种测试土壤类型都表现出 CE 活性,这突出表明土壤适合作为生产正己酸酯的理想来源。与厌氧污泥和坑泥相比,原生土壤 CE 系统表现出更高的选择性(60.53%)、专一性(82.32%)、碳分布(60.00%)、电子传递效率(165.00%)和电导率(0.59 ms∙cm-1)。动力学分析进一步证实了土壤在缩短滞后时间和提高产量方面的优势。微生物群落分析表明,假单胞菌、氮单胞菌和梭状芽孢杆菌的相对丰度与正己酸酯产量呈正相关。此外,元基因组学分析表明,关键微生物物种中的功能基因丰度较高,直接揭示了正己酸酯形成的途径,包括原位二氧化碳利用、乙醇氧化、脂肪酸生物合成(FAB)和反向β-氧化(RBO)。FAB和RBO的众多功能主要与假单胞菌、梭状芽孢杆菌、罗杜球菌、司来诺单胞菌和革囊菌有关,这表明这些菌属可能参与了CE过程或与之相关。总之,这种创新的接种策略为正己酸酯的生产提供了一个高效的微生物源,强调了在厌氧土壤微生物生态学中考虑CE活性的重要性,并通过土壤联合体的探索为巨大的经济和环境效益提供了潜力。
Chain Elongation Using Native Soil Inocula: Exceptional n-Caproate Biosynthesis Performance and Microbial Mechanisms
This study demonstrates the feasibility and effectiveness of utilizing native soils as a resource for inocula to produce n-caproate through the chain elongation (CE) platform, offering new insights into anaerobic soil processes. The results reveal that all five of the tested soil types exhibit CE activity when supplied with high concentrations of ethanol and acetate, highlighting the suitability of soil as an ideal source for n-caproate production. Compared with anaerobic sludge and pit mud, the native soil CE system exhibited higher selectivity (60.53%), specificity (82.32%), carbon distribution (60.00%), electron transfer efficiency (165.00%), and conductivity (0.59 ms∙cm−1). Kinetic analysis further confirmed the superiority of soil in terms of a shorter lag time and higher yield. A microbial community analysis indicated a positive correlation between the relative abundances of Pseudomonas, Azotobacter, and Clostridium and n-caproate production. Moreover, metagenomics analysis revealed a higher abundance of functional genes in key microbial species, providing direct insights into the pathways involved in n-caproate formation, including in situ CO2 utilization, ethanol oxidation, fatty acid biosynthesis (FAB), and reverse beta-oxidation (RBO). The numerous functions in FAB and RBO are primarily associated with Pseudomonas, Clostridium, Rhodococcus, Stenotrophomonas, and Geobacter, suggesting that these genera may play roles that are involved or associated with the CE process. Overall, this innovative inoculation strategy offers an efficient microbial source for n-caproate production, underscoring the importance of considering CE activity in anaerobic soil microbial ecology and holding potential for significant economic and environmental benefits through soil consortia exploration.
期刊介绍:
Engineering, an international open-access journal initiated by the Chinese Academy of Engineering (CAE) in 2015, serves as a distinguished platform for disseminating cutting-edge advancements in engineering R&D, sharing major research outputs, and highlighting key achievements worldwide. The journal's objectives encompass reporting progress in engineering science, fostering discussions on hot topics, addressing areas of interest, challenges, and prospects in engineering development, while considering human and environmental well-being and ethics in engineering. It aims to inspire breakthroughs and innovations with profound economic and social significance, propelling them to advanced international standards and transforming them into a new productive force. Ultimately, this endeavor seeks to bring about positive changes globally, benefit humanity, and shape a new future.