分离尚未培养的微生物--自由基清除剂的应用。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2024-09-04 Epub Date: 2024-02-28 DOI:10.2323/jgam.2024.02.002
Yōko Takahashi
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引用次数: 0

摘要

从土壤样本中分离出的主要细菌菌株的培养滤液可增加用于分离未培养细菌的琼脂平板上的微生物菌落数。培养滤液中导致菌落数增加的因素之一是超氧化物歧化酶(SOD)。在传统的实验室琼脂培养基中检测到了活性氧(O2-、H2O2 和 ・OH)的生成。使用添加了自由基清除剂(SOD、过氧化氢酶、抗坏血酸或芦丁)的琼脂培养基能有效增加从土壤样本中生长出来的微生物菌株的菌落数和种类。对这些分离菌株的分类研究发现了放线菌门的新分类群;新描述了一个科、三个属和九个种。其中一株属于新的棒状杆菌科的闽东棒状杆菌 KV-614T 是在添加了 SOD 的琼脂培养基上分离出来的。P. minatonensis KV-614T 代表了放线菌门(Actinomycetota)中的一个新品系。聚合酶链式反应(PCR)研究使用特异性引物来检测与 Solirubrobacterales 目 Patulibacter 属相关的菌株,结果显示其分布频率很高,在 70% 以上的土壤样本中都能检测到。这些数据表明,使用自由基清除剂可能有助于分离出土壤中广泛分布的一些尚未培养的微生物。
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Isolation of hitherto-uncultivated microorganisms- Application of radical scavengers.

The culture filtrates of the predominant bacterial strains isolated from soil samples have been shown to increase the microbial colony counts on agar plates used for the isolation of uncultured bacteria. One of the factors in the culture filtrates responsible for this increase was identified to be superoxide dismutase (SOD). The generation of reactive oxygen species (O2-, H2O2, and ・OH) was detected from conventional laboratory agar media. The use of agar media supplemented with radical scavengers (SOD, catalase, ascorbic acid, or rutin) effectively increased the colony counts and kinds of microbial strains that grew from soil samples. Taxonomical studies on these isolates revealed new taxa for phylum Actinomycetota; one family, three genera, and nine species were newly described. One of the strains, Patulibacter minatonensis KV-614T belonging to the new family Patulibacteraceae, was isolated on agar medium supplemented with SOD. P. minatonensis KV-614T represents a novel lineage within the phylum Actinomycetota. A polymerase chain reaction (PCR) study using specific primers for the detection of strains related to the genus Patulibacter, order Solirubrobacterales, showed a high distribution frequency, with detection in over 70% of the soil samples tested. These data suggest that the use of radical scavengers may facilitate the isolation of some hitherto-uncultivated microorganisms widely distributed in soil.

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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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