基于形态特征和新开发的 EST-SSR 和 SRAP 标记的泰国保留桑种质的遗传多样性

IF 2.4 3区 农林科学 Q1 Agricultural and Biological Sciences Horticulture Environment and Biotechnology Pub Date : 2024-03-06 DOI:10.1007/s13580-024-00599-2
Nutdanai Putthisawong, Surapa Nutthapornnitchakul, Natnicha Thumthuan, Chatuporn Kuleung, Athipat Ngernmuen, Piyama Tasanasuwan, Chatchawan Jantasuriyarat
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引用次数: 0

摘要

桑树(Morus L.)是养蚕业的重要作物,是桑蚕的主要食物来源。泰国养蚕业历史悠久,引进并改良了许多本土栽培品种,创造出新的杂交后代。了解这些栽培品种的遗传差异对于保护它们并在选育中加以利用至关重要。在本研究中,利用 SRAP 和 EST-SSR 标记对泰国具有代表性的 85 个桑树品种进行了形态学观察和遗传关系分析。研究结果表明,泰国桑树的形态特征足以区分M. macroura Miq.和野生杂交桑树,以及由M. alba L.和M. australis Poir.及其杂交种组成的群体。12 个 SRAP 引物组合产生了 193 个多态性扩增子,平均每个引物组有 17.0 条带,PIC 平均值为 0.259。11 个新型 EST-SSR 引物产生了 35 个扩增子,平均每个引物组有 3.2 个等位基因,平均 PIC 为 0.139。用 R studio 中的 UPGMA 算法绘制的树枝图显示,野生桑和野生杂交桑与本文研究的驯化桑在遗传上有一定的距离。这些发现对泰国桑树种质的特征描述、改良、分子系统学和保护具有重要意义。
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Genetic diversity of Thailand reserved mulberry germplasm based on morphological characteristics and newly developed EST-SSR and SRAP markers

Mulberry (Morus L.) is an important crop for the sericulture industry, serving as the primary food source for the silkworm Bombyx mori L. Thailand has a long history of practicing sericulture and has imported and improved upon many indigenous cultivars to create new hybrid offspring. It is crucial to understand the genetic divergence of these accessions for their conservation and utilization in selection and breeding. In this study, 85 representative mulberry accessions in Thailand were observed morphology and analyzed for their genetic relationships using SRAP and EST-SSR markers. The findings indicate that the morphological traits of Thai mulberry are distinctive enough to differentiate between M. macroura Miq. and wild hybrid mulberry, and a group consisting of M. alba L. and M. australis Poir., and their hybrids. 12 SRAP primer combinations produced 193 polymorphic amplicons with an average of 17.0 bands per primer set, and the mean of PIC was 0.259. Eleven novel EST-SSR primers generated 35 amplicons with an average of 3.2 alleles per primer set, and the average PIC was 0.139. The dendrogram obtained using the UPGMA algorithm in R studio showed that the wild and wild hybrid mulberry were genetically distant from the domesticated species studied here. These findings have important implications for the characterization, improvement, molecular systematics, and conservation of Thai mulberry germplasm.

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来源期刊
Horticulture Environment and Biotechnology
Horticulture Environment and Biotechnology Agricultural and Biological Sciences-Horticulture
CiteScore
4.30
自引率
4.20%
发文量
0
审稿时长
6 months
期刊介绍: Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science". HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI. The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.
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