新生儿接触邻苯二甲酸二(2-乙基己酯)后性腺细胞发育受到干扰。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-03-10 DOI:10.1016/j.repbio.2024.100877
Estefanía Reyes-Cruz , Julio César Rojas-Castañeda , Daniel Adrian Landero-Huerta , Norma Hernández-Jardón , Rafael Reynoso-Robles , María de Lourdes Juárez-Mosqueda , Alfredo Medrano , Rosa María Vigueras-Villaseñor
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引用次数: 0

摘要

实验动物在产前和/或产后服用邻苯二甲酸二(2-乙基己酯)(DEHP)会导致精子发生改变。然而,DEHP 影响生育能力的机制尚不清楚,可能是通过改变生殖腺细胞的存活和分化。本研究旨在评估新生小鼠单次摄入 DEHP 对生殖腺细胞增殖、分化和存活的影响,及其对曲细精管上皮细胞和精子质量的长期影响。实验将 BALB/c 小鼠分为对照组和 DEHP 组。DEHP 组的每只小鼠在出生时均被注射单剂量 500 mg/Kg。在产后 1、2、4、6、8、10 和 70 天(dpp)对动物进行分析。对睾丸组织进行形态学分析,以确定不同类型的性腺细胞、分化指数、曲细精管上皮改变以及Stra8、Pcna和Vimentin蛋白的免疫反应。在 70 dpp 时对动物的曲细精管上皮和精子质量进行了长期评估。DEHP 动物组出现了性腺细胞变性和分化延迟,导致精原细胞数量减少(Stra8 +)、细胞增殖(Pcna +)和波形蛋白丝紊乱。这些事件对曲细精管上皮和精液质量产生了长期影响。我们的研究表明,睾丸在出生后的一段时间内对接触DEHP极为敏感,这会导致性腺细胞变性和分化延迟。这种情况会对曲细精管上皮的质量和精子参数产生长期或永久的影响。
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Disruption of gonocyte development following neonatal exposure to di (2-ethylhexyl) phthalate

Pre- and/or post-natal administrations of di(2-ethylhexyl) phthalate (DEHP) in experimental animals cause alterations in the spermatogenesis. However, the mechanism by which DEHP affects fertility is unknown and could be through alterations in the survival and differentiation of the gonocytes. The aim of the present study was to evaluate the effect of a single administration of DEHP in newborn mice on gonocytic proliferation, differentiation and survival and its long-term effects on seminiferous epithelium and sperm quality. BALB/c mice distributed into Control and DEHP groups were used. Each animal in the DEHP group was given a single dose of 500 mg/Kg at birth. The animals were analyzed at 1, 2, 4, 6, 8, 10 and 70 days postpartum (dpp). Testicular tissues were processed for morphological analysis to determine the different types of gonocytes, differentiation index, seminiferous epithelial alterations, and immunoreactivity to Stra8, Pcna and Vimentin proteins. Long-term evaluation of the seminiferous epithelium and sperm quality were carried out at 70 dpp. The DEHP animal group presented gonocytic degeneration with delayed differentiation, causing a reduction in the population of spermatogonia (Stra8 +) in the cellular proliferation (Pcna+) and disorganization of Vimentin filaments. These events had long-term repercussions on the quality of the seminiferous epithelium and semen. Our study demonstrates that at birth, there is a period that the testes are extremely sensitive to DEHP exposure, which leads to gonocytic degeneration and delay in their differentiation. This situation can have long-term repercussions or permanent effects on the quality of the seminiferous epithelium and sperm parameters.

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ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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