Evelin Carine Alves Silva, Jéssica Arielli Pradelli, Guilherme Ferreira da Silva, Paulo Sérgio Cerri, Mario Tanomaru-Filho, Juliane Maria Guerreiro-Tanomaru
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Unstained sections were evaluated under polarized light and by immunohistochemistry for osteocalcin (OCN). Data were submitted to two-way <span>anova</span> followed by Tukey's test (<i>p ≤</i> .05), except for OCN. OCN data were submitted to Kruskal–Wallis and Dunn and Friedman <i>post hoc</i> tests followed by the Nemenyi test at a significance level of 5%.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>At 7, 15, and 30 days, thick capsules containing numerous ICs were seen around the materials. At 60 days, a moderate inflammatory reaction was observed for NPutty, BC while MTA HP presented thin capsules with moderate inflammatory cells. In all periods, NPutty specimens contained the highest values of ICs (<i>p <</i> .05). From 7 to 60 days, the number of ICs reduced significantly while an increase in the number of fibroblasts and birefringent collagen content was observed. At 7 and 15 days, no significant difference was observed in the immunoexpression of OCN (<i>p ></i> .05). At 30 and 60 days, NPutty showed the lowest values of OCN (<i>p <</i> .05). At 60 days, a similar immunoexpression was observed for BC and MTA HP (<i>p ></i> .05). In all time intervals, capsules around NPutty, BC, and MTA HP showed von Kossa-positive and birefringent structures.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Despite the greater inflammatory reaction promoted by NeoPutty than BC and MTA HP, the reduction in the thickness of capsules, the increase in the number of fibroblasts, and the reduction in the number of ICs indicate that this bioceramic material is biocompatible Furthermore, NeoPutty presents the ability to induce mineralization activity.</p>\n </section>\n </div>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biocompatibility and bioactive potential of NeoPUTTY calcium silicate-based cement: An in vivo study in rats\",\"authors\":\"Evelin Carine Alves Silva, Jéssica Arielli Pradelli, Guilherme Ferreira da Silva, Paulo Sérgio Cerri, Mario Tanomaru-Filho, Juliane Maria Guerreiro-Tanomaru\",\"doi\":\"10.1111/iej.14054\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Aim</h3>\\n \\n <p>To evaluate the inflammatory reaction and the ability to induce mineralization activity of a new repair material, NeoPUTTY (NPutty; NuSmile, USA), in comparison with Bio-C Repair (BC; Angelus, Brazil) and MTA Repair HP (MTA HP; Angelus, Brazil).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methodology</h3>\\n \\n <p>Polyethylene tubes were filled with materials or kept empty (control group, CG) and implanted in subcutaneous tissue of rats for 7, 15, 30, and 60 days (<i>n</i> = 6/group). 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引用次数: 0
摘要
目的：评估一种新型修复材料 NeoPUTTY（NPutty；NuSmile，美国）与 Bio-C Repair（BC；Angelus，巴西）和 MTA Repair HP（MTA HP；Angelus，巴西）的炎症反应和诱导矿化活性的能力：方法：在聚乙烯管中填充材料或保持空管（对照组，CG），并植入大鼠皮下组织 7、15、30 和 60 天（n = 6/组）。进行蒴果厚度、炎症细胞（IC）数量、成纤维细胞、胶原蛋白含量和 von Kossa 分析。未染色的切片在偏振光下进行评估，并通过免疫组织化学检测骨钙素（OCN）。除 OCN 外，其他数据均进行双向 anova 后的 Tukey's 检验（p ≤ .05）。OCN数据进行Kruskal-Wallis、Dunn和Friedman事后检验，然后进行Nemenyi检验，显著性水平为5%：7天、15天和30天时，材料周围出现了含有大量集成电路的厚囊。在 60 天时，NPutty 和 BC 出现中度炎症反应，而 MTA HP 则出现带有中度炎症细胞的薄囊。在所有时期，NPutty 试样的 IC 值最高（p .05）。在 30 天和 60 天时，NPutty 的 OCN 值最低（p .05）。在所有时间间隔内，NPutty、BC 和 MTA HP 周围的胶囊都显示出 von Kossa 阳性和双折射结构：尽管与 BC 和 MTA HP 相比，NeoPutty 引发的炎症反应更大，但囊肿厚度的减少、成纤维细胞数量的增加以及 IC 数量的减少都表明这种生物陶瓷材料具有生物相容性。
Biocompatibility and bioactive potential of NeoPUTTY calcium silicate-based cement: An in vivo study in rats
Aim
To evaluate the inflammatory reaction and the ability to induce mineralization activity of a new repair material, NeoPUTTY (NPutty; NuSmile, USA), in comparison with Bio-C Repair (BC; Angelus, Brazil) and MTA Repair HP (MTA HP; Angelus, Brazil).
Methodology
Polyethylene tubes were filled with materials or kept empty (control group, CG) and implanted in subcutaneous tissue of rats for 7, 15, 30, and 60 days (n = 6/group). Capsule thickness, number of inflammatory cells (ICs), fibroblasts, collagen content, and von Kossa analysis were performed. Unstained sections were evaluated under polarized light and by immunohistochemistry for osteocalcin (OCN). Data were submitted to two-way anova followed by Tukey's test (p ≤ .05), except for OCN. OCN data were submitted to Kruskal–Wallis and Dunn and Friedman post hoc tests followed by the Nemenyi test at a significance level of 5%.
Results
At 7, 15, and 30 days, thick capsules containing numerous ICs were seen around the materials. At 60 days, a moderate inflammatory reaction was observed for NPutty, BC while MTA HP presented thin capsules with moderate inflammatory cells. In all periods, NPutty specimens contained the highest values of ICs (p < .05). From 7 to 60 days, the number of ICs reduced significantly while an increase in the number of fibroblasts and birefringent collagen content was observed. At 7 and 15 days, no significant difference was observed in the immunoexpression of OCN (p > .05). At 30 and 60 days, NPutty showed the lowest values of OCN (p < .05). At 60 days, a similar immunoexpression was observed for BC and MTA HP (p > .05). In all time intervals, capsules around NPutty, BC, and MTA HP showed von Kossa-positive and birefringent structures.
Conclusions
Despite the greater inflammatory reaction promoted by NeoPutty than BC and MTA HP, the reduction in the thickness of capsules, the increase in the number of fibroblasts, and the reduction in the number of ICs indicate that this bioceramic material is biocompatible Furthermore, NeoPutty presents the ability to induce mineralization activity.
期刊介绍：
The International Endodontic Journal is published monthly and strives to publish original articles of the highest quality to disseminate scientific and clinical knowledge; all manuscripts are subjected to peer review. Original scientific articles are published in the areas of biomedical science, applied materials science, bioengineering, epidemiology and social science relevant to endodontic disease and its management, and to the restoration of root-treated teeth. In addition, review articles, reports of clinical cases, book reviews, summaries and abstracts of scientific meetings and news items are accepted.
The International Endodontic Journal is essential reading for general dental practitioners, specialist endodontists, research, scientists and dental teachers.
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